Document Detail


The effect of MK-801 on cortical spreading depression in the penumbral zone following focal ischaemia in the rat.
MedLine Citation:
PMID:  1314840     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cortical spreading depression (CSD) is a transient depression of neuronal activity that spreads across the cortical surface. In the present studies, we have investigated CSD activity in the penumbral zone following permanent middle cerebral artery (MCA) occlusion in the rat (n = 16/group), using double-barreled Ca(2+)-sensitive microelectrodes. Measurements of CSD activity were made for 3 h in each animal. During this time, a varying number of spontaneous CSDs were seen in the control group (total was 30, with a range of 0-7/rat). These CSDs were of varying duration: "small" (approximately 1 min) and "big" (5-45 min) CSDs. During a CSD, the extracellular [Ca2+] decreased to 0.11 +/- 0.07 mM (mean +/- SD). After 3 h, the extracellular [Ca2+] in the cortex (penumbral zone) was either normal (10/16 rats) or lowered to 0.5 mM (2/16 rats) or to 0.1 mM (4/16 rats). In the caudate nucleus (ischaemic core area), all rats had an extracellular [Ca2+] of approximately 0.1 mM when measured after the 3 h recording period. Neuropathological evaluation of the brains of the animals, which had been allowed to survive for 24 h after MCA occlusion, revealed ischaemic damage in the dorsolateral cortex and caudate nucleus. Administration of the noncompetitive NMDA antagonist, MK-801 (3 mg/kg i.p.), 30 min after MCA occlusion resulted in 24 and 29% reductions in the volume of hemispheric and cortical damage, respectively, which was highly significant (p less than 0.0001); no protection was seen against caudate damage.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
R Gill; P Andiné; L Hillered; L Persson; H Hagberg
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  12     ISSN:  0271-678X     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-05-27     Completed Date:  1992-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  371-9     Citation Subset:  IM    
Affiliation:
Institute of Neurobiology, University of Göteborg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Ischemia / drug therapy*
Calcium / analysis
Cerebral Arterial Diseases / drug therapy*
Cerebral Cortex / drug effects*,  pathology
Dizocilpine Maleate / pharmacology*
Male
Rats
Rats, Inbred Strains
Synaptic Transmission / drug effects*
Tissue Survival / drug effects
Chemical
Reg. No./Substance:
7440-70-2/Calcium; 77086-22-7/Dizocilpine Maleate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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