Document Detail

The effect of L-arginine on slow motility of mammalian outer hair cell.
MedLine Citation:
PMID:  12684177     Owner:  NLM     Status:  MEDLINE    
The effect of L-arginine on the slow motility of mammalian cochlear outer hair cells was studied in this experiment. L-Arginine (3 mM) but not D-arginine (3 mM) or other amino acids (L-aspartate or L-glutamate) induced length increases of guinea pig outer hair cell. Similarly, the membrane-permeant cGMP analogues, 8-(4-chlorophenylthio)guanosine 3':5'-cyclic monophosphate (1 mM) or 8-bromo-guanosine 3':5'-cyclic monophosphate (1 mM) induced length increases of guinea pig outer hair cells. These length increases induced by L-arginine can be attenuated by a 30 min preincubation of the cells with the nitric oxide synthase inhibitors N(G)-nitro-L-arginine methyl ester hydrochloride (3 mM) or 7-nitroindazole (1 mM). Comparing the effects of L-arginine and ionomycin on cell length and intracellular calcium change in outer hair cells, both L-arginine and ionomycin were able to induce the elongation of outer hair cells but L-arginine did not change the fluorescence intensity of Fluo-3. Preincubation of the cells with EGTA (3 mM) for 40 min to reduce the extracellular calcium concentration did not influence the effect of L-arginine. This experiment demonstrated that nitric oxide/cGMP pathway involvement in regulating the slow motility of mammalian outer hair cells cannot be ruled out. The effect of L-arginine is independent of extracellular calcium concentration.
Min-Jon Lin; Mao-Chang Su; Ching-Ting Tan; Ching-Chyuan Su; Shuan-Yow Li; Ruey-Hseng Lin; Shoei-Yn Lin-Shiau; Chui-Chen Hung; Shiann-Yann Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hearing research     Volume:  178     ISSN:  0378-5955     ISO Abbreviation:  Hear. Res.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-09     Completed Date:  2003-12-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7900445     Medline TA:  Hear Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  52-8     Citation Subset:  IM    
Department of Life Sciences, Chung Shan Medical University, Taichung City, Taiwan.
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MeSH Terms
Arginine / pharmacology*
Calcium / metabolism
Cell Membrane Permeability
Cell Movement / drug effects
Cell Size / drug effects
Cyclic GMP / analogs & derivatives,  pharmacokinetics
Enzyme Inhibitors / pharmacology
Extracellular Fluid / metabolism
Guinea Pigs
Hair Cells, Auditory, Outer / cytology,  drug effects,  physiology*
Indazoles / pharmacology
Intracellular Membranes / drug effects,  metabolism
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors
Osmolar Concentration
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Indazoles; 2942-42-9/7-nitroindazole; 50903-99-6/NG-Nitroarginine Methyl Ester; 74-79-3/Arginine; 7440-70-2/Calcium; 7665-99-8/Cyclic GMP; EC Oxide Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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