| The effect of L-arginine on slow motility of mammalian outer hair cell. | |
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MedLine Citation:
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PMID: 12684177 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of L-arginine on the slow motility of mammalian cochlear outer hair cells was studied in this experiment. L-Arginine (3 mM) but not D-arginine (3 mM) or other amino acids (L-aspartate or L-glutamate) induced length increases of guinea pig outer hair cell. Similarly, the membrane-permeant cGMP analogues, 8-(4-chlorophenylthio)guanosine 3':5'-cyclic monophosphate (1 mM) or 8-bromo-guanosine 3':5'-cyclic monophosphate (1 mM) induced length increases of guinea pig outer hair cells. These length increases induced by L-arginine can be attenuated by a 30 min preincubation of the cells with the nitric oxide synthase inhibitors N(G)-nitro-L-arginine methyl ester hydrochloride (3 mM) or 7-nitroindazole (1 mM). Comparing the effects of L-arginine and ionomycin on cell length and intracellular calcium change in outer hair cells, both L-arginine and ionomycin were able to induce the elongation of outer hair cells but L-arginine did not change the fluorescence intensity of Fluo-3. Preincubation of the cells with EGTA (3 mM) for 40 min to reduce the extracellular calcium concentration did not influence the effect of L-arginine. This experiment demonstrated that nitric oxide/cGMP pathway involvement in regulating the slow motility of mammalian outer hair cells cannot be ruled out. The effect of L-arginine is independent of extracellular calcium concentration. |
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Authors:
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Min-Jon Lin; Mao-Chang Su; Ching-Ting Tan; Ching-Chyuan Su; Shuan-Yow Li; Ruey-Hseng Lin; Shoei-Yn Lin-Shiau; Chui-Chen Hung; Shiann-Yann Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hearing research Volume: 178 ISSN: 0378-5955 ISO Abbreviation: Hear. Res. Publication Date: 2003 Apr |
Date Detail:
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Created Date: 2003-04-09 Completed Date: 2003-12-11 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7900445 Medline TA: Hear Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 52-8 Citation Subset: IM |
Affiliation:
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Department of Life Sciences, Chung Shan Medical University, Taichung City, Taiwan. mjl@csmu.edu.tw |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / pharmacology* Calcium / metabolism Cell Membrane Permeability Cell Movement / drug effects Cell Size / drug effects Cyclic GMP / analogs & derivatives, pharmacokinetics Enzyme Inhibitors / pharmacology Extracellular Fluid / metabolism Guinea Pigs Hair Cells, Auditory, Outer / cytology, drug effects, physiology* Indazoles / pharmacology Intracellular Membranes / drug effects, metabolism NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide Synthase / antagonists & inhibitors Osmolar Concentration |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Indazoles; 2942-42-9/7-nitroindazole; 50903-99-6/NG-Nitroarginine Methyl Ester; 74-79-3/Arginine; 7440-70-2/Calcium; 7665-99-8/Cyclic GMP; EC 1.14.13.39/Nitric Oxide Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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