Document Detail


The effect of 5 days of aspartate and asparagine supplementation on glucose transport activity in rat muscle.
MedLine Citation:
PMID:  19821260     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The consumption of protein supplements containing amino acids is increasing around the world. Aspartate (Asp) and asparagine (Asn) are amino acids metabolized by skeletal muscle. This metabolism involves biochemical pathways that are involved in increasing Krebs cycle activity via anaplerotic reactions, resulting in higher glutamine concentrations. A connection between amino acid supplementation, glycogen concentration, and glucose uptake has been previously demonstrated. The purpose of this study was to evaluate the effect of Asp and Asn supplementation on glucose uptake in rats using three different glycogen concentrations. The results indicate that Asp and Asn supplementation in rats with high glycogen concentrations (fed state) further increased the glycogen concentration in the muscle, and decreased in vitro 2-deoxyglucose (a glucose analog) uptake by the muscle at maximal insulin concentrations. When animals had a medium glycogen concentration (consumed lard for 3 days), glucose uptake was higher in the supplemented group at sub-maximal insulin concentrations. We conclude that supplementation of Asp and Asn reduced glucose transport in rat muscle only at higher levels of glycogen. The ingestion of lard for 3 days changed the responsiveness and sensitivity to insulin, and that group had higher levels of insulin sensitivity with Asp and Asn supplementation.
Authors:
Antonio H Lancha; Jacques R Poortmans; Luciana O Pereira
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  27     ISSN:  1099-0844     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-02     Completed Date:  2010-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  552-7     Citation Subset:  IM    
Affiliation:
Biodynamic Department, Physical Education and Sport School, University of S?o Paulo, S?o Paulo, Brazil. lanchajr@usp.br
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MeSH Terms
Descriptor/Qualifier:
Animals
Asparagine / administration & dosage*
Aspartic Acid / administration & dosage*
Biological Transport
Dietary Supplements*
Glucose / metabolism*
Glycogen / metabolism
Insulin / metabolism
Male
Muscle, Skeletal / metabolism*
Random Allocation
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
11061-68-0/Insulin; 50-99-7/Glucose; 56-84-8/Aspartic Acid; 7006-34-0/Asparagine; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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