Document Detail

The effect of 1,25-dihydroxyvitamin D3 on the relationship between growth and differentiation in HL-60 cells.
MedLine Citation:
PMID:  3469486     Owner:  NLM     Status:  MEDLINE    
Cells of the human promyelocytic leukaemia line HL-60 may be induced to differentiate along the monocytic lineage by the seco-steroid hormone 1,25-dihydroxyvitamin D3. We used fluorescence-activated cell sorting to show that, in addition to inducing differentiation, the hormone caused HL-60 cells to accumulate in G1/G0 phases of the cell cycle. We also labelled differentiating cells with both a monocyte-specific antibody and a DNA stain simultaneously. Analysis of these cell populations showed that, although cells acquired the differentiated phenotype irrespective of their position in the cell cycle, they eventually became arrested in G1/G0 as a consequence of differentiation. The normal relationship between differentiation and growth which is lost on malignant transformation is therefore restored by treatment of HL-60 cells with 1,25-dihydroxyvitamin D3.
C P Daniel; A Parreira; J M Goldman; D M McCarthy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia research     Volume:  11     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  1987  
Date Detail:
Created Date:  1987-04-01     Completed Date:  1987-04-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  191-6     Citation Subset:  IM    
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MeSH Terms
Calcitriol / pharmacology*
Cell Differentiation / drug effects
Cell Division / drug effects
Cell Line
Flow Cytometry
Leukemia, Myeloid, Acute / pathology*
Reg. No./Substance:

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