| The effect of 1,25-dihydroxyvitamin D3 on the relationship between growth and differentiation in HL-60 cells. | |
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MedLine Citation:
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PMID: 3469486 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells of the human promyelocytic leukaemia line HL-60 may be induced to differentiate along the monocytic lineage by the seco-steroid hormone 1,25-dihydroxyvitamin D3. We used fluorescence-activated cell sorting to show that, in addition to inducing differentiation, the hormone caused HL-60 cells to accumulate in G1/G0 phases of the cell cycle. We also labelled differentiating cells with both a monocyte-specific antibody and a DNA stain simultaneously. Analysis of these cell populations showed that, although cells acquired the differentiated phenotype irrespective of their position in the cell cycle, they eventually became arrested in G1/G0 as a consequence of differentiation. The normal relationship between differentiation and growth which is lost on malignant transformation is therefore restored by treatment of HL-60 cells with 1,25-dihydroxyvitamin D3. |
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Authors:
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C P Daniel; A Parreira; J M Goldman; D M McCarthy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Leukemia research Volume: 11 ISSN: 0145-2126 ISO Abbreviation: Leuk. Res. Publication Date: 1987 |
Date Detail:
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Created Date: 1987-04-01 Completed Date: 1987-04-01 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7706787 Medline TA: Leuk Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 191-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calcitriol
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pharmacology* Cell Differentiation / drug effects Cell Division / drug effects Cell Line Flow Cytometry Humans Interphase Leukemia, Myeloid, Acute / pathology* |
| Chemical | |
Reg. No./Substance:
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32222-06-3/Calcitriol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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