| T20/DP178, an ectodomain peptide of human immunodeficiency virus type 1 gp41, is an activator of human phagocyte N-formyl peptide receptor. | |
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MedLine Citation:
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PMID: 10339497 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human immunodeficiency virus type 1 (HIV-1) envelope protein gp41 mediates viral fusion with human host cells. The peptide segment T20/DP178, located in the C-terminus of the ectodomain of gp41, interacts with the N-terminal leucine zipper-like domain on gp41 to establish the fusogenic conformation of the virus. Synthetic T20/DP178 peptide is highly efficacious in inhibiting HIV-1 infection in vitro by disrupting the transformation of fusogenic status of viral gp41; thus, it has been proposed for clinical trial. We report that synthetic T20/DP178 is a chemoattractant and activator of human peripheral blood phagocytes but not of T lymphocytes. We further demonstrate that T20/DP178 specifically activates a seven-transmembrane, G-protein-coupled phagocyte receptor for N-formylated chemotactic peptides, formyl peptide receptor (FPR). Moreover, synthetic T20/DP178 analogs lacking N-terminal amino acids acted as FPR antagonists. Our results suggest that gp41 peptides regulate phagocyte function via FPR and identify a novel mechanism by which HIV-1 may modulate innate immunity. |
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Authors:
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S B Su; W H Gong; J L Gao; W P Shen; M C Grimm; X Deng; P M Murphy; J J Oppenheim; J M Wang |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Blood Volume: 93 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-06-24 Completed Date: 1999-06-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3885-92 Citation Subset: AIM; IM; X |
Affiliation:
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Laboratory of Molecular Immunoregulation, Division of Basic Sciences, and Intramural Research Support Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured HIV Envelope Protein gp41 / metabolism*, pharmacology HIV Infections / virology HIV-1 / physiology Humans Monocytes / physiology*, virology* Neutrophils / physiology*, virology* Peptide Fragments / metabolism*, pharmacology Phagocytosis* / drug effects Receptors, Formyl Peptide Receptors, Immunologic / physiology* Receptors, Peptide / physiology* Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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N01-CO-56000/CO/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/HIV Envelope Protein gp41; 0/Peptide Fragments; 0/Receptors, Formyl Peptide; 0/Receptors, Immunologic; 0/Receptors, Peptide; 0/peptide T20 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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