Document Detail

The ectodermal control in chick limb development: Wnt-7a, Shh, Bmp-2 and Bmp-4 expression and the effect of FGF-4 on gene expression.
MedLine Citation:
PMID:  9025066     Owner:  NLM     Status:  MEDLINE    
We have manipulated the chick limb bud by dorsoventrally inverting the ectoderm, by grafting the AER to the dorsal or ventral ectoderm and by insertion of an FGF-4 soaked heparin bead into the mesoderm. After dorso-ventral reversal of the ectoderm, Wnt-7a expression is autonomous from an early stage of limb development in the original dorsal ectoderm. Exogenous FGF-4 causes ectopic Wnt-7a expression and induces ectopic Shh. In addition, exogenous FGF-4 increases the thickness of cartilages and also shortens them, and both Bmp-2 and Bmp-4 may mediate this effect. The ectoderm outside the AER can regulate not only the dorso-ventral polarity of the underlying mesenchyme cells but also the cartilage formation, and both Bmp-2 and Bmp-4 may mediate this control.
K Akita; P Francis-West; N Vargesson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mechanisms of development     Volume:  60     ISSN:  0925-4773     ISO Abbreviation:  Mech. Dev.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-06-23     Completed Date:  1997-06-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9101218     Medline TA:  Mech Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  127-37     Citation Subset:  IM    
Department of Anatomy and Developmental Biology, University College, London, UK.
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MeSH Terms
Avian Proteins*
Body Patterning / drug effects
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins / genetics*
Cartilage / drug effects,  embryology
Chick Embryo
Ectoderm / drug effects,  metabolism
Fibroblast Growth Factor 4
Fibroblast Growth Factors / pharmacology*
Gene Expression Regulation, Developmental / drug effects
Hedgehog Proteins
In Situ Hybridization
Limb Buds / drug effects,  embryology*,  metabolism*,  surgery
Proteins / genetics*
Proto-Oncogene Proteins / genetics*,  pharmacology*
Time Factors
Transforming Growth Factor beta*
Wnt Proteins
Reg. No./Substance:
0/Avian Proteins; 0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Protein 4; 0/Bone Morphogenetic Proteins; 0/Fibroblast Growth Factor 4; 0/Hedgehog Proteins; 0/Proteins; 0/Proto-Oncogene Proteins; 0/Trans-Activators; 0/Transforming Growth Factor beta; 0/WNT7A protein, Gallus gallus; 0/Wnt Proteins; 62031-54-3/Fibroblast Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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