Document Detail

The eclipse period of Escherichia coli.
MedLine Citation:
PMID:  11080169     Owner:  NLM     Status:  MEDLINE    
The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation. The SeqA protein was absolutely required for the eclipse, and DnaA titration studies suggested that the SeqA protein prevented the binding of multiple DnaA molecules on oriC (initial complex formation). No correlation between the amount of SeqA and eclipse length was revealed, but increased SeqA levels affected chromosome partitioning and/or cell division. This was corroborated further by an aberrant nucleoid distribution in SeqA-deficient cells. We suggest that the SeqA protein's role in maintaining the eclipse is tied to a function in chromosome organization.
U von Freiesleben; M A Krekling; F G Hansen; A Løbner-Olesen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  19     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-15     Completed Date:  2001-01-04     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  6240-8     Citation Subset:  IM    
Department of Microbiology, The Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark.
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MeSH Terms
Bacterial Outer Membrane Proteins
Bacterial Proteins / genetics,  metabolism
Base Sequence
Cell Cycle
Cell Division
Chromosomes, Bacterial / metabolism
DNA Primers / genetics
DNA Replication
DNA-Binding Proteins / metabolism
Escherichia coli / cytology,  genetics,  metabolism*
Escherichia coli Proteins
Origin Recognition Complex
Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism
Transcription Factors*
Viral Proteins
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/DNA Primers; 0/DNA-Binding Proteins; 0/DnaA protein, Bacteria; 0/Escherichia coli Proteins; 0/OriC chromosomal replication origin; 0/Origin Recognition Complex; 0/SeqA protein, E coli; 0/Transcription Factors; 0/Viral Proteins; EC methyltransferase; EC DNA-Methyltransferase (Adenine-Specific); EC protein, E coli

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