| The ecdysone inducible gene expression system: unexpected effects of muristerone A and ponasterone A on cytokine signaling in mammalian cells. | |
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MedLine Citation:
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PMID: 11399528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inducible gene expression systems in mammalian cells have been shown to be valuable processes to study the specific function of a protein in differentiation, proliferation or survival/apoptosis. Usually, these systems use as inducible reagents, compounds that are thought to be neutral and devoid of physiological or biologically undesirable effects in mammalian cells. We recently used the ecdysone inducible gene expression system in hematopoietic cells and found that the two inducer analogs of ecdysone, muristerone A and ponasterone A, altered the signaling pathways induced by IL-3 in the pro-B cell-line, Ba/F3. Indeed, we showed that these two analogs potentiate the IL-3-dependent activation of the PI 3-kinase/Akt pathway, which could ultimately interfere with the growth, and/or survival of these cells. |
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Authors:
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S Constantino; R Santos; S Gisselbrecht; F Gouilleux |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European cytokine network Volume: 12 ISSN: 1148-5493 ISO Abbreviation: Eur. Cytokine Netw. Publication Date: 2001 Apr-Jun |
Date Detail:
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Created Date: 2001-06-11 Completed Date: 2001-09-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9100879 Medline TA: Eur Cytokine Netw Country: France |
Other Details:
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Languages: eng Pagination: 365-7 Citation Subset: IM |
Affiliation:
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Institut Cochin de Génétique Moléculaire, Institut National de la Santé et de la Recherche Médicale U. 363, Hôpital Cochin, 27, rue du Faubourg-Saint-Jacques, 75014 Paris, France. |
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism Animals Cell Line Ecdysterone / analogs & derivatives*, pharmacology* Enzyme Activation Gene Expression Regulation / drug effects* Interleukin-3 / metabolism* Phosphorylation Protein-Serine-Threonine Kinases* Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-akt Signal Transduction / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-3; 0/Proto-Oncogene Proteins; 13408-56-5/ponasterone A; 38778-30-2/muristerone A; 5289-74-7/Ecdysterone; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/Proto-Oncogene Proteins c-akt |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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