Document Detail

The ecdysone inducible gene expression system: unexpected effects of muristerone A and ponasterone A on cytokine signaling in mammalian cells.
MedLine Citation:
PMID:  11399528     Owner:  NLM     Status:  MEDLINE    
Inducible gene expression systems in mammalian cells have been shown to be valuable processes to study the specific function of a protein in differentiation, proliferation or survival/apoptosis. Usually, these systems use as inducible reagents, compounds that are thought to be neutral and devoid of physiological or biologically undesirable effects in mammalian cells. We recently used the ecdysone inducible gene expression system in hematopoietic cells and found that the two inducer analogs of ecdysone, muristerone A and ponasterone A, altered the signaling pathways induced by IL-3 in the pro-B cell-line, Ba/F3. Indeed, we showed that these two analogs potentiate the IL-3-dependent activation of the PI 3-kinase/Akt pathway, which could ultimately interfere with the growth, and/or survival of these cells.
S Constantino; R Santos; S Gisselbrecht; F Gouilleux
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European cytokine network     Volume:  12     ISSN:  1148-5493     ISO Abbreviation:  Eur. Cytokine Netw.     Publication Date:    2001 Apr-Jun
Date Detail:
Created Date:  2001-06-11     Completed Date:  2001-09-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9100879     Medline TA:  Eur Cytokine Netw     Country:  France    
Other Details:
Languages:  eng     Pagination:  365-7     Citation Subset:  IM    
Institut Cochin de Génétique Moléculaire, Institut National de la Santé et de la Recherche Médicale U. 363, Hôpital Cochin, 27, rue du Faubourg-Saint-Jacques, 75014 Paris, France.
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MeSH Terms
1-Phosphatidylinositol 3-Kinase / metabolism
Cell Line
Ecdysterone / analogs & derivatives*,  pharmacology*
Enzyme Activation
Gene Expression Regulation / drug effects*
Interleukin-3 / metabolism*
Protein-Serine-Threonine Kinases*
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-akt
Signal Transduction / drug effects*
Reg. No./Substance:
0/Interleukin-3; 0/Proto-Oncogene Proteins; 13408-56-5/ponasterone A; 38778-30-2/muristerone A; 5289-74-7/Ecdysterone; EC 3-Kinase; EC Kinases; EC Proteins c-akt

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