| An early circulating factor in severe sepsis modulates apoptosis of monocytes and lymphocytes. | |
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MedLine Citation:
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PMID: 21030664 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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We hypothesized that a factor may circulate in serum early during sepsis, modulating apoptosis of monocytes and lymphocytes. Serum was collected from 20 healthy volunteers and from 48 patients with severe sepsis/shock within 12 h from signs of the first failing organ. PBMCs were isolated from 20 healthy volunteers and incubated with collected sera. Apoptosis and expression of CD95 were determined by flow cytometry; experiments were run in the presence of caspase-8 and caspase-9 inhibitors and of CaCl(2). Activity of caspase-3 was determined in cell lysates by a chromogenic kinetic assay. Incubation with serum of patients induced apoptosis of CD4 lymphocytes and inhibited apoptosis of CD14 monocytes. This was attenuated after diluting serum or mixing with healthy serum. Activity of caspase-3 was consistent with these findings. Induced apoptosis of CD4 lymphocytes was greater among nonsurvivors, and it was inhibited in the presence of caspase inhibitors. Inhibitors did not modify the effect of patients' serum on apoptosis of CD14 monocytes. CaCl(2) reversed the inhibitory effect on apoptosis of CD14 moncytes. The above findings support the hypothesis for the existence of an early circulating factor in severe sepsis/shock, modulating apoptosis of CD4 lymphocytes and of CD14 monocytes by interaction with the two apoptotic pathways. |
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Authors:
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Ilia Vaki; Hariklia Kranidioti; Vassiliki Karagianni; Aikaterini Spyridaki; Antigone Kotsaki; Christina Routsi; Evangelos J Giamarellos-Bourboulis |
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Publication Detail:
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Type: Journal Article Date: 2010-10-28 |
Journal Detail:
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Title: Journal of leukocyte biology Volume: 89 ISSN: 1938-3673 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 343-9 Citation Subset: IM |
Affiliation:
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1.1 Rimini St., 124 62 Athens, Greece. giamarel@ath.forthnet.gr. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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