Document Detail


An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells.
MedLine Citation:
PMID:  19015217     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pathogenicity of various Streptomyces scabies isolates involved in potato scab disease was correlated with the production of thaxtomin A. Since calcium is known as an essential second messenger associated with pathogen-induced plant responses and cell death, it was investigated whether thaxtomin A could induce a Ca2+ influx related to cell death and to other putative plant responses using Arabidopsis thaliana suspension cells, which is a convenient model to study plant-microbe interactions. A. thaliana cells were treated with micromolar concentrations of thaxtomin A. Cell death was quantified and ion flux variations were analysed from electrophysiological measurements with the apoaequorin Ca2+ reporter protein and by external pH measurement. Involvement of anion and calcium channels in signal transduction leading to programmed cell death was determined by using specific inhibitors. These data suggest that this toxin induces a rapid Ca2+ influx and cell death in A. thaliana cell suspensions. Moreover, these data provide strong evidence that the Ca2+ influx induced by thaxtomin A is necessary to achieve this cell death and is a prerequisite to early thaxtomin A-induced responses: anion current increase, alkalization of the external medium, and the expression of PAL1 coding for a key enzyme of the phenylpropanoid pathway.
Authors:
R Errakhi; A Dauphin; P Meimoun; A Lehner; D Reboutier; P Vatsa; J Briand; K Madiona; J P Rona; M Barakate; D Wendehenne; C Beaulieu; F Bouteau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-17
Journal Detail:
Title:  Journal of experimental botany     Volume:  59     ISSN:  1460-2431     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2008  
Date Detail:
Created Date:  2008-11-27     Completed Date:  2008-12-31     Revised Date:  2009-08-28    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  4259-70     Citation Subset:  IM    
Affiliation:
LEM (EA 3514), Université Paris Diderot-Paris7, 2, place Jussieu, F-75251 Paris cedex 05, France.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / drug effects*,  genetics,  physiology*
Biological Transport
Calcium / metabolism*
Cell Death / drug effects
Cell Membrane / drug effects,  genetics,  metabolism
Indoles / pharmacology*
Ion Channels / genetics,  metabolism
Piperazines / pharmacology*
Plant Proteins / genetics,  metabolism
Signal Transduction / drug effects
Streptomyces / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Indoles; 0/Ion Channels; 0/Piperazines; 0/Plant Proteins; 122380-18-1/thaxtomine A; 7440-70-2/Calcium
Comments/Corrections
Comment In:
Plant Signal Behav. 2009 Feb;4(2):142-4   [PMID:  19649193 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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