Document Detail


eChickAtlas: an introduction to the database.
MedLine Citation:
PMID:  23355415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The precise control of gene expression is critical in embryonic development. Quantitative assays, such as microarrays and RNA sequencing, provide gene expression levels for a large number of genes, but do not contain spatial information. In contrast, in situ methods, such as in situ hybridization and immunohistochemistry, provide spatial resolution, but poor quantification and can only reveal the expression of one, or very few genes at a time. Furthermore, the usual methods of documenting the results, by photographing whole mounts or sections, makes it very difficult to assess the three-dimensional (3D) relationships between expressing and nonexpressing cells. Optical projection tomography (OPT) can capture the full 3D expression pattern in a whole embryo at a reasonable level of resolution and at moderately high throughput. A large database containing spatio-temporal patterns of expression for the mouse (e-Mouse Atlas Project, EMAP, www.emouseatlas.org) has been created, incorporating 3D information. Like the mouse, the chick is an important model in developmental biology and translational studies. To facilitate comparisons between these important model organisms, we have created a 3D anatomical atlas, accompanied by an anatomical ontology of the chick embryo and a database of gene expression patterns during chick development. This database is publicly available (www.echickatlas.org).
Authors:
Frances Wong; Monique C M Welten; Claire Anderson; Andrew A Bain; Jiahui Liu; Michael N Wicks; Gordana Pavlovska; Megan G Davey; Paula Murphy; Duncan Davidson; Cheryll A Tickle; Claudio D Stern; Richard A Baldock; David W Burt
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Publication Detail:
Type:  Journal Article     Date:  2013-03-30
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  51     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-24     Completed Date:  2013-12-31     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  365-71     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chick Embryo
Chickens / genetics*
Computational Biology / methods
Databases, Genetic*
Gene Expression Regulation*
Genomics / methods*
Internet
Software
Grant Support
ID/Acronym/Agency:
BB/G000972/1//Biotechnology and Biological Sciences Research Council; MR/K001744/1//Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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