| Dynamics of zeaxanthin binding to the photosystem II monomeric antenna protein Lhcb6 (CP24) and modulation of its photoprotection properties. | |
| | |
MedLine Citation:
|
PMID: 20494647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lhcb6 (CP24) is a monomeric antenna protein of photosystem II, which has been shown to play special roles in photoprotective mechanisms, such as the Non-Photochemical Quenching and reorganization of grana membranes in excess light conditions. In this work we analyzed Lhcb6 in vivo and in vitro: we show this protein, upon activation of the xanthophyll cycle, accumulates zeaxanthin into inner binding sites faster and to a larger extent than any other pigment-protein complex. By comparative analysis of Lhcb6 complexes violaxanthin or zeaxanthin binding, we demonstrate that zeaxanthin not only down-regulates chlorophyll singlet excited states, but also increases the efficiency of chlorophyll triplet quenching, with consequent reduction of singlet oxygen production and significant enhancement of photo-stability. On these bases we propose that Lhcb6, the most recent addition to the Lhcb protein family which evolved concomitantly to the adaptation of photosynthesis to land environment, has a crucial role in zeaxanthin-dependent photoprotection. |
| | |
Authors:
|
Nico Betterle; Matteo Ballottari; Rainer Hienerwadel; Luca Dall'Osto; Roberto Bassi |
Related Documents
:
|
21258647 - Self-interaction chromatography of proteins on a microfluidic monolith. 17157187 - Identification of 4-hydroxynonenal-modified retinal proteins induced by photooxidative ... 6705917 - Sulfhydryl group modification of photoreceptor g-protein prevents its light-induced bin... 3298237 - Optically detected magnetic resonance of tryptophan residues in escherichia coli ssb ge... 1907727 - Protein analysis of theileria sergenti/buffeli/orientalis piroplasms by two-dimensional... 19995727 - The chlamydomonas chloroplast hlp protein is required for nucleoid organization and gen... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-28 |
Journal Detail:
|
Title: Archives of biochemistry and biophysics Volume: 504 ISSN: 1096-0384 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-10-19 Completed Date: 2010-11-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
|
Languages: eng Pagination: 67-77 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Dipartimento di Biotecnologie, Università di Verona, Ca' Vignal 1, Strada le Grazie 15, I-37134 Verona, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Arabidopsis
/
cytology,
metabolism*,
radiation effects* Arabidopsis Proteins / metabolism* Chlorophyll / metabolism Epoxy Compounds / chemistry Kinetics Light* Light-Harvesting Protein Complexes / metabolism* Photobleaching Photosystem II Protein Complex / chemistry*, metabolism Plant Leaves / metabolism, radiation effects Protein Binding Singlet Oxygen / metabolism Spectrum Analysis Thylakoids / metabolism Xanthophylls / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Arabidopsis Proteins; 0/Epoxy Compounds; 0/LHCB5 protein, Arabidopsis; 0/Lhcb6 protein, Arabidopsis; 0/Light-Harvesting Protein Complexes; 0/Photosystem II Protein Complex; 0/Xanthophylls; 126-29-4/violaxanthin; 1406-65-1/Chlorophyll; 144-68-3/zeaxanthin; 17778-80-2/Singlet Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Catalase evolved to concentrate H(2)O(2) at its active site.
Next Document: Cellular pathways for transport and efflux of ascorbate and dehydroascorbate.