Document Detail


Dynamics of zeaxanthin binding to the photosystem II monomeric antenna protein Lhcb6 (CP24) and modulation of its photoprotection properties.
MedLine Citation:
PMID:  20494647     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lhcb6 (CP24) is a monomeric antenna protein of photosystem II, which has been shown to play special roles in photoprotective mechanisms, such as the Non-Photochemical Quenching and reorganization of grana membranes in excess light conditions. In this work we analyzed Lhcb6 in vivo and in vitro: we show this protein, upon activation of the xanthophyll cycle, accumulates zeaxanthin into inner binding sites faster and to a larger extent than any other pigment-protein complex. By comparative analysis of Lhcb6 complexes violaxanthin or zeaxanthin binding, we demonstrate that zeaxanthin not only down-regulates chlorophyll singlet excited states, but also increases the efficiency of chlorophyll triplet quenching, with consequent reduction of singlet oxygen production and significant enhancement of photo-stability. On these bases we propose that Lhcb6, the most recent addition to the Lhcb protein family which evolved concomitantly to the adaptation of photosynthesis to land environment, has a crucial role in zeaxanthin-dependent photoprotection.
Authors:
Nico Betterle; Matteo Ballottari; Rainer Hienerwadel; Luca Dall'Osto; Roberto Bassi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-28
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  504     ISSN:  1096-0384     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-19     Completed Date:  2010-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-77     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Dipartimento di Biotecnologie, Università di Verona, Ca' Vignal 1, Strada le Grazie 15, I-37134 Verona, Italy.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / cytology,  metabolism*,  radiation effects*
Arabidopsis Proteins / metabolism*
Chlorophyll / metabolism
Epoxy Compounds / chemistry
Kinetics
Light*
Light-Harvesting Protein Complexes / metabolism*
Photobleaching
Photosystem II Protein Complex / chemistry*,  metabolism
Plant Leaves / metabolism,  radiation effects
Protein Binding
Singlet Oxygen / metabolism
Spectrum Analysis
Thylakoids / metabolism
Xanthophylls / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Epoxy Compounds; 0/LHCB5 protein, Arabidopsis; 0/Lhcb6 protein, Arabidopsis; 0/Light-Harvesting Protein Complexes; 0/Photosystem II Protein Complex; 0/Xanthophylls; 126-29-4/violaxanthin; 1406-65-1/Chlorophyll; 144-68-3/zeaxanthin; 17778-80-2/Singlet Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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