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On the dynamics of the microfilament system in HeLa cells.
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MedLine Citation:
PMID:  6802855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We measured the pools of unpolymerized and filamentous actin in homogenates of HeLa cells made in several different lysis buffers, as well as after treatment of cells with a variety of chemicals or trypsin, and after adenovirus (type 2) infection. This was possible when a series of factors concerning the basic culture conditions were kept constant: e.g., serum type used, serum batch, cell density, time after subcultivation of cells, and buffering substance in the medium. Homogenates from untreated cells usually contain 35-45 percent of the total actin in an unpolymerized form. With some batches of cells this number can be as high as 50 percent. In sparse cultures (3 x 10(4) cell/cm(2)), HeLa cells contain approximately 10 pg actin/cell, while the corresponding number is only 5 pg in dense cultures (3 x 10(5) cells/cm(2)). Treatment of cells with cytochalasin B increases the pool of unpolymerized actin by approximately 30-40 percent, while colchicine decreases the fraction of unpolymerized actin by 20 percent. The oxidant diamide increases the filamentous actin pool 25-50 percent. Glucose, sodium azide, dinitrophenol, serum starvation, or thymidine treatment does not affect the distribution between unpolymerized and filamentous actin to any significant extent. Trypsin and EDTA induced rounding up of cells but did not change the actin distribution. The distribution of actin between G- and F-forms was unchanged after adenovirus infection. These results show that significant changes in the actin pools can be induced in nucleated cells. However, several treatments which alter the morphology and motility of cells are not accompanied by an alteration in the G-/F-actin ratio.
Authors:
I Blikstad; L Carlsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  93     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1982 Apr 
Date Detail:
Created Date:  1982-06-21     Completed Date:  1982-06-21     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  122-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Colchicine / pharmacology
Cytochalasin B / pharmacology
Cytoskeleton / ultrastructure*
Detergents / pharmacology
Diamide / pharmacology
Edetic Acid / pharmacology
Hela Cells / drug effects,  ultrastructure
Humans
Kinetics
Octoxynol
Polyethylene Glycols / pharmacology
Trypsin / pharmacology
Chemical
Reg. No./Substance:
0/Actins; 0/Detergents; 0/Polyethylene Glycols; 10465-78-8/Diamide; 14930-96-2/Cytochalasin B; 60-00-4/Edetic Acid; 64-86-8/Colchicine; 9002-93-1/Octoxynol; EC 3.4.21.4/Trypsin
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 4 Year: 1982
Volume: 93 Issue: 1
First Page: 122 Last Page: 128
ID: 2112103
Publisher Id: 931122
PubMed Id: 6802855

On the dynamics of the microfilament system in HeLa cells
I Blikstad
L Carlsson


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