Document Detail


A dynamic cell adhesion surface regulates tissue architecture in growth plate cartilage.
MedLine Citation:
PMID:  24764078     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The architecture and morphogenetic properties of tissues are founded in the tissue-specific regulation of cell behaviors. In endochondral bones, the growth plate cartilage promotes bone elongation via regulated chondrocyte maturation within an ordered, three-dimensional cell array. A key event in the process that generates this cell array is the transformation of disordered resting chondrocytes into clonal columns of discoid proliferative cells aligned with the primary growth vector. Previous analysis showed that column-forming chondrocytes display planar cell divisions, and the resulting daughter cells rearrange by ∼90° to align with the lengthening column. However, these previous studies provided limited information about the mechanisms underlying this dynamic process. Here we present new mechanistic insights generated by application of a novel time-lapse confocal microscopy method along with immunofluorescence and electron microscopy. We show that, during cell division, daughter chondrocytes establish a cell-cell adhesion surface enriched in cadherins and β-catenin. Rearrangement into columns occurs concomitant with expansion of this adhesion surface in a process more similar to cell spreading than to migration. Column formation requires cell-cell adhesion, as reducing cadherin binding via chelation of extracellular calcium inhibits chondrocyte rearrangement. Importantly, physical indicators of cell polarity, such as cell body alignment, are not prerequisites for oriented cell behavior. Our results support a model in which regulation of adhesive surface dynamics and cortical tension by extrinsic signaling modifies the thermodynamic landscape to promote organization of daughter cells in the context of the three-dimensional growth plate tissue.
Authors:
Sarah M Romereim; Nicholas H Conoan; Baojiang Chen; Andrew T Dudley
Related Documents :
23801048 - β-galactosyl-pyrrolidinyl diazeniumdiolate: an efficient tool to investigate nitric oxi...
23296668 - Monitoring oncogenic b-raf-induced senescence in melanocytes.
24618778 - Surface plasmon resonance for cell-based clinical diagnosis.
23633848 - Influence of limonin on wnt signalling molecule in hepg2 cell lines.
2503608 - Influence of agents that affect intracellular calcium regulation on progesterone secret...
10927038 - Human testicular mast cells contain tryptase: increased mast cell number and altered di...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-4-24
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  -     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease.
Next Document:  Co-activation of microRNAs by Zelda is essential for early Drosophila development.