| The dynamic behavior of chemically "stiffened" red blood cells in microchannel flows. | |
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MedLine Citation:
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PMID: 20303993 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rigidity of red blood cells (RBCs) plays an important role in whole blood viscosity and is correlated with several cardiovascular diseases. Two chemical agents that are commonly used to study cell deformation are diamide and glutaraldehyde. Despite diamide's common usage, there are discrepancies in the literature surrounding diamide's effect on the deformation of RBCs in shear and pressure-driven flows; in particular, shear flow experiments have shown that diamide stiffens cells, while pressure-driven flow in capillaries did not give this result. We performed pressure-driven flow experiments with RBCs in a microfluidic constriction and quantified the cell dynamics using high-speed imaging. Diamide, which affects RBCs by cross-linking spectrin skeletal membrane proteins, did not reduce deformation and showed an unchanged effective strain rate when compared to healthy cells. In contrast, glutaraldehyde, which is a non-specific fixative that acts on all components of the cell, did reduce deformation and showed increased instances of tumbling, both of which are characteristic features of stiffened, or rigidified, cells. Because glutaraldehyde increases the effective viscosity of the cytoplasm and lipid membrane while diamide does not, one possible explanation for our results is that viscous effects in the cytoplasm and/or lipid membrane are a dominant factor in dictating dynamic responses of RBCs in pressure-driven flows. Finally, literature on the use of diamide as a stiffening agent is summarized, and provides supporting evidence for our conclusions. |
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Authors:
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Alison M Forsyth; Jiandi Wan; William D Ristenpart; Howard A Stone |
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Publication Detail:
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Type: Journal Article Date: 2010-03-18 |
Journal Detail:
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Title: Microvascular research Volume: 80 ISSN: 1095-9319 ISO Abbreviation: Microvasc. Res. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-01 Completed Date: 2010-12-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0165035 Medline TA: Microvasc Res Country: United States |
Other Details:
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Languages: eng Pagination: 37-43 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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School of Engineering and Applied Sciences, Harvard University, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cytoplasm
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drug effects Diamide / pharmacology Elasticity / drug effects Erythrocyte Deformability / drug effects Erythrocyte Membrane / drug effects Erythrocytes / cytology*, drug effects Glutaral / pharmacology Hemorheology / drug effects* Humans Microfluidic Analytical Techniques Microfluidics* Motion Viscosity / drug effects |
| Chemical | |
Reg. No./Substance:
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10465-78-8/Diamide; 111-30-8/Glutaral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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