Document Detail


A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff.
MedLine Citation:
PMID:  6167536     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines an alkaline phosphatase-labeled concanavalin A-5-bromo-3-indolyl phosphate, p-toluidine salt (Con A-ALP-BIPT) method with periodic acid-Schiff (PAS) sequence. With the present dual staining method, it is possible to color alpha-D-glucosyl and alpha-D-mannosyl residues blue and 1,2-glycol groups of neutral complex carbohydrates magenta. The validity of this method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.
Authors:
K Yamada; H Kitamura; Y Maseki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  71     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1981  
Date Detail:
Created Date:  1981-10-14     Completed Date:  1981-10-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  513-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase*
Animals
Carbohydrates / analysis*
Cattle
Chickens
Concanavalin A*
Dogs
Haplorhini
Histocytochemistry*
Humans
Microscopy / methods
Periodic Acid-Schiff Reaction*
Rats
Staining and Labeling
Swine
Chemical
Reg. No./Substance:
0/Carbohydrates; 11028-71-0/Concanavalin A; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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