Document Detail

The dual-specific binding of dengue virus and target cells for the antibody-dependent enhancement of dengue virus infection.
MedLine Citation:
PMID:  16493039     Owner:  NLM     Status:  MEDLINE    
Using flow cytometric assay and monoclonal anti-dengue Ab, we observed that both anti-E and anti-prM Abs could enhance dengue virus infection in a concentration-dependent but serotype-independent manner. Increases were found in both the percentage of dengue-infected cells and the expression of dengue E and NS1 protein per cell. Dengue virion binding and infection were enhanced on FcR-bearing cells via the Fc-FcgammaRII pathway. Furthermore, anti-prM Ab also enhanced dengue virion binding and infection on cells lacking FcR, such as BHK-21 or A549 cells, by the mechanism of peptide (CPFLKQNEPEDIDCW)-specific binding. Anti-prM Ab cross-reacted with BHK-21 or A549 cells and recognized self-Ags such as heat shock protein 60. In summary, a novel mechanism of anti-prM Ab-mediated enhancement on dengue virus infection was found to be mediated by dual specific binding to dengue virion and to target cells, in addition to the traditional enhancement on FcR-bearing cells.
Kao-Jean Huang; Yu-Ching Yang; Yee-Shin Lin; Jyh-Hsiung Huang; Hsiao-Sheng Liu; Trai-Ming Yeh; Shun-Hua Chen; Ching-Chuan Liu; Huan-Yao Lei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  176     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-22     Completed Date:  2006-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2825-32     Citation Subset:  AIM; IM    
Department of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
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MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal / physiology*
Antibodies, Viral / physiology*
B-Lymphocytes / immunology,  virology
Binding Sites / immunology
Cell Line
Dendritic Cells / immunology,  virology
Dengue / immunology*,  virology*
Dengue Virus / immunology*,  metabolism
Jurkat Cells
K562 Cells
Leukemia P388
Macrophages / immunology,  virology
Mice, Inbred BALB C
Molecular Sequence Data
NIH 3T3 Cells
Peptide Fragments / genetics,  immunology
Receptors, Fc / immunology
U937 Cells
Viral Envelope Proteins / immunology
Viral Proteins / immunology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/E protein, Dengue virus type 3; 0/Peptide Fragments; 0/Receptors, Fc; 0/Viral Envelope Proteins; 0/Viral Proteins; 0/prM protein, Dengue virus type 3

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