Document Detail


A dried preparation of liposomes containing muramyl tripeptide phosphatidylethanolamine as a potent activator of human blood monocytes to the antitumor state.
MedLine Citation:
PMID:  3731204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies were performed on the activation of human blood monocytes to the antitumor state by a dried preparation of multilamellar vesicle (MLV) liposomes in which synthetic muramyl tripeptide phosphatidylethanolamine (MTP-PE) was inserted directly into the liposome membrane. Dried liposomes composed of synthetic phospholipids [phosphatidylcholine (PC) and phosphatidylserine (PS) in a molar ratio of 7:3] were prepared by lyophilization. Dried liposome-MTP-PE was found to be superior in several ways to free desmethyl muramyl dipeptide (norMDP) or conventional liposome-MTP-PE, prepared immediately before use. First, dried liposome-MTP-PE was stable and strongly activated monocytes when stored for over 3 months in a freezer at -20 degrees C or even in suspension at 4 degrees C. Second, human monocytes in suspension, as well as in the adherent form, were activated to the tumoricidal state by interaction for at least 4 h with the dried preparation of liposome-MTP-PE. Third, monocytes activated with the dried liposome-MTP-PE or conventionally prepared liposome-MTP-PE maintained their tumoricidal activity for a longer period (4 days) than those activated with free norMDP. These results indicate that the dried preparation of liposome-MTP-PE can be stored for a long time, has a reproducible effect that can be standardized and should be valuable for in situ activation of human monocytes to the tumoricidal state, which is associated with eradication of cancer metastases.
Authors:
S Sone; T Utsugi; P Tandon; M Ogawara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer immunology, immunotherapy : CII     Volume:  22     ISSN:  0340-7004     ISO Abbreviation:  Cancer Immunol. Immunother.     Publication Date:  1986  
Date Detail:
Created Date:  1986-09-17     Completed Date:  1986-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605732     Medline TA:  Cancer Immunol Immunother     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  191-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylmuramyl-Alanyl-Isoglutamine / administration & dosage,  analogs & derivatives*,  pharmacology
Cytotoxicity, Immunologic / drug effects*
Drug Storage
Humans
Liposomes / administration & dosage*
Monocytes / drug effects,  immunology*
Neoplasms / immunology*,  therapy
Phosphatidylethanolamines / administration & dosage*,  pharmacology
Suspensions
Time Factors
Chemical
Reg. No./Substance:
0/Liposomes; 0/Phosphatidylethanolamines; 0/Suspensions; 53678-77-6/Acetylmuramyl-Alanyl-Isoglutamine; 83461-56-7/muramylNAc-Ala-isoGln-Lys-tripeptide-PE

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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