| A double knockout of two putative multicopper oxidase genes in Pseudomonas putida GB-1 eliminates manganese (II, III) oxidation. | |
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MedLine Citation:
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PMID: 23124227 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Bacterial manganese(II) oxidation impacts the redox cycling of Mn, elements and other compounds in the environment, thus the mechanisms of and enzymes responsible for Mn(II) oxidation are important to understand. In several Mn(II)-oxidizing organisms, the identified Mn(II) oxidase belongs to either the multi-copper oxidase (MCO) or the heme peroxidase family of proteins. However, the identity of the oxidase in Pseudomonas putida GB-1 has long remained unknown. To identify the P. putida GB-1 oxidase enzyme, we searched its genome and found several homologues to known or suspected Mn(II) oxidase-encoding genes (mnxG, mofA, moxA, and mopA). To narrow this list, we assumed the Mn(II) oxidase gene would be conserved among Mn(II)-oxidizing pseudomonads but not in non-oxidizers and performed a genome comparison to eleven Pseudomonas species. We further assumed that the oxidase gene would be regulated by MnxR, a transcription factor required for Mn(II) oxidation. Two loci met all these criteria: PputGB1_2447, which encodes an MCO homologous to MnxG and PputGB1_2665, which encodes an MCO with very low homology to MofA. In-frame deletions of either loci resulted in strains that retained some ability to oxidize Mn(II) or Mn(III); loss of oxidation was only attained upon deletion of both genes. These results suggest that 2447 and 2665 encode two MCO enzymes that each independently are capable of oxidizing both Mn(II) and Mn(III). The purpose of this redundancy is unclear; however, differences in oxidation phenotype for the single mutants suggest specialization in function for the two enzymes. |
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Authors:
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Kati Geszvain; James K McCarthy; Bradley M Tebo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-2 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Division of Environmental and Biomolecular Systems Institute of Environmental Health Oregon Health & Science University 20000 NW Walker Rd Beaverton, OR 97006-8921. |
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