| A double blind, single centre, sub-chronic reperfusion trial evaluating FX06 following haemorrhagic shock in pigs. | |
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MedLine Citation:
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PMID: 19058891 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Haemorrhagic shock causes ischaemia and subsequent fluid resuscitation causes reperfusion injury, jointly resulting in high morbidity and mortality. We tested whether the anti-inflammatory fibrin-derived peptide, Bbeta(15-42), also called FX06, is tissue protective in a model of haemorrhagic shock. METHODS: In a pig model, we standardised the severity of haemorrhagic shock by achieving a cumulative oxygen deficit of approximately 100ml/kg body weight by withdrawing blood over a period of 1h. This was followed by resuscitation with shed blood and full electrolyte solution, and pigs were monitored for 3 days. At reperfusion, 17 pigs were randomly assigned to FX06 or solvent treatment. RESULTS: FX06-treated pigs demonstrated improved cardiac function (stroke volume index: 67ml/m(2) versus 33ml/m(2)), decreased troponin T release in the early reperfusion (0.24ng/ml versus 0.78ng/ml), decreased AST levels after 24h (106U/l versus 189U/l) and decreased creatinine levels after 24h (108micromol/l versus 159micromol/l). Furthermore, FX06-treated pigs demonstrated preservation of the gut/blood barrier, while controls demonstrated high endotoxin plasma levels indicating translocation of bacteria and/or its products (0.2EU/ml versus 24.3EU/ml) after 24h. This study also demonstrates a significantly improved neurological performance in the FX06 group as determined by S100beta serum levels (0.72microg/l versus 1.25microg/l) after 48h and neurological deficit scores (11 versus 70) after 24h. CONCLUSION: FX06 - when administered as an adjunct to fluid resuscitation therapy - is organ protective in pigs. Further investigations are warranted to reveal the protective mechanism of FX06. |
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Authors:
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Jan P Roesner; Peter Petzelbauer; Alexander Koch; Nguyen Tran; Thomas Iber; Christian Mutz; Brigitte Vollmar; Gabriele E F Nöldge-Schomburg; Kai Zacharowski |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-05 |
Journal Detail:
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Title: Resuscitation Volume: 80 ISSN: 0300-9572 ISO Abbreviation: Resuscitation Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-19 Completed Date: 2009-05-15 Revised Date: 2009-08-25 |
Medline Journal Info:
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Nlm Unique ID: 0332173 Medline TA: Resuscitation Country: Ireland |
Other Details:
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Languages: eng Pagination: 264-71 Citation Subset: IM |
Affiliation:
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Department of Anaesthesia and Intensive Care Medicine, University Hospital Rostock, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticoagulants / pharmacology* Aspartate Aminotransferases / blood Blood Glucose / analysis Blood Urea Nitrogen Creatine Kinase / blood Disease Models, Animal Double-Blind Method Drug Evaluation, Preclinical Endotoxins / blood Fibrin Fibrinogen Degradation Products / pharmacology* Glutamate Dehydrogenase / blood Interleukins / blood L-Lactate Dehydrogenase / blood Leukocyte Count Male Nerve Growth Factors / blood Neurologic Examination Oxygen / blood Peptide Fragments / pharmacology* Pulmonary Gas Exchange Random Allocation Reperfusion / methods* Resuscitation S100 Proteins / blood Shock, Hemorrhagic / drug therapy* Stroke Volume / drug effects Swine Troponin T / blood Tumor Necrosis Factor-alpha / blood |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Blood Glucose; 0/Endotoxins; 0/Fibrin Fibrinogen Degradation Products; 0/Interleukins; 0/Nerve Growth Factors; 0/Peptide Fragments; 0/S-100 calcium-binding protein beta subunit; 0/S100 Proteins; 0/Troponin T; 0/Tumor Necrosis Factor-alpha; 0/fibrinogen Bbeta (15-42); 7782-44-7/Oxygen; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.4.1.2/Glutamate Dehydrogenase; EC 2.6.1.1/Aspartate Aminotransferases; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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