Document Detail


dl-alpha-tocopherol induces apoptosis in erythroleukemia, prostate, and breast cancer cells.
MedLine Citation:
PMID:  9200147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vitamin E, best known as a potent antioxidant, has been shown to have other functions that are not mediated by this activity. Recent reports have suggested that vitamin E may inhibit smooth muscle cell and also cancer cell growth. We have studied the effect of dl-alpha-tocopherol (vitamin E) on a series of well-established cancer cell lines that included two erythroleukemia cell lines and a hormone-responsive breast and prostate cancer cell line. Cell proliferation was examined in these cell lines, which were maintained at optimal growth conditions. A dose-dependent inhibition of cell growth was found in all cell lines examined, with the MCF-7 breast and CRL-1740 prostate cancer cell lines showing potent suppression of growth at 0.1 mM vitamin E, whereas the erythroleukemia cell lines, HEL and OCIM-1, responded only at > 0.25 mM vitamin E with inhibition of proliferation. Studies of [3H]thymidine incorporation showed that vitamin E supplementation reduced DNA synthesis in all cell lines. Analysis of high-molecular-weight DNA revealed extensive fragmentation, indicating apoptosis of all cell lines supplemented with vitamin E. Our studies thus give evidence of a general inhibition of cell proliferation by dl-alpha-tocopherol, with breast and prostate cancer cells distinctly more sensitive than erythroleukemia cells.
Authors:
G Sigounas; A Anagnostou; M Steiner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nutrition and cancer     Volume:  28     ISSN:  0163-5581     ISO Abbreviation:  Nutr Cancer     Publication Date:  1997  
Date Detail:
Created Date:  1997-08-08     Completed Date:  1997-08-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  30-5     Citation Subset:  IM    
Affiliation:
Division of Hematology/Oncology, East Carolina University School of Medicine, Greenville, NC 27858, USA. sigounas@brody.med.ecu.edu
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Breast Neoplasms / pathology*
Cell Division / drug effects
DNA / biosynthesis
DNA Fragmentation
Electrophoresis, Agar Gel
Humans
Leukemia, Erythroblastic, Acute / pathology*
Male
Prostatic Neoplasms / pathology*
Tumor Cells, Cultured
Vitamin E / pharmacology*
Grant Support
ID/Acronym/Agency:
HL-39109/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
1406-18-4/Vitamin E; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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