| The division during bacterial sporulation is symmetrically located in Sporosarcina ureae. | |
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MedLine Citation:
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PMID: 9350865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunofluorescence microscopy was used to visualize the FtsZ band that marks the site of septation in Sporosarcina ureae. Image analysis indicated that the vegetative division was symmetrically located with respect to the ends of the cells. Fusions of lacZ to the sporulation loci, spollA and cotE, of Bacillus subtilis were introduced into S. ureae by mobilization of plasmids containing the fusions from Escherichia coli. The fusions showed similar patterns of sporulation-associated expression in S. ureae to those observed in B. subtilis. Formation of beta-galactosidase encoded by the spollA-lacZ fusion made it possible to identify early sporulating cells by immunofluorescence microscopy. Analysis of the position of FtsZ bands in cells expressing spollA-lacZ indicated that the location of sporulation division was symmetrical with respect to the ends of the cells, in sharp contrast to the asymmetrical location of septation in sporulating Bacilli. It is inferred that asymmetry of location of the sporulation division is not essential for the compartmentalization of gene expression that follows the division. |
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Authors:
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L Zhang; M L Higgins; P J Piggot |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular microbiology Volume: 25 ISSN: 0950-382X ISO Abbreviation: Mol. Microbiol. Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1998-02-19 Completed Date: 1998-02-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1091-8 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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genetics Bacterial Proteins / analysis*, genetics Cell Division Cytoskeletal Proteins* Gene Expression Genes, Bacterial Gram-Positive Cocci / growth & development, metabolism, physiology* Image Processing, Computer-Assisted Lac Operon Microscopy, Fluorescence Plasmids Spores, Bacterial |
| Grant Support | |
ID/Acronym/Agency:
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GM43577/GM/NIGMS NIH HHS; GM51335/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/CotE protein, Bacillus subtilis; 0/Cytoskeletal Proteins; 0/FtsZ protein, Bacteria |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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