Document Detail


The diurnal variation of epidermal metabolism.
MedLine Citation:
PMID:  6890085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In previous studies, the rates of epidermal glycolysis and amino acid incorporation were found to parallel rates of mitosis in both the hypoplasia associated with starvation and the hyperplasia produced by treatment with hexadecane. To examine whether similar changes in epidermal metabolism accompany the known diurnal variation in epidermal mitotic activity, the rates of glycolysis and incorporation of amino acids in epidermal sheets from skin samples at various times during a 24-hr period were assayed. These activities were found to parallel the diurnal variation of mitosis demonstrated by metaphase counts. Diurnal variation of metabolism in the suprabasal layers was investigated by examining the relative rates of incorporation of histidine and leucine: it was found that histidine showed a broader peak of activity than that seen for the other measured activities. When epidermal samples were prepared form tissue treated with hexadecane to induce hyperplasia, an increase in metabolic activity and decrease in the amplitude of diurnal variation was observed. The results indicate that the general metabolic activities of the epidermis follow a diurnal pattern similar to that found for mitosis.
Authors:
R R Harris; I C Mackenzie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  79     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1982-12-18     Completed Date:  1982-12-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  283-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Animals
Cell Division
Circadian Rhythm*
Epidermis / metabolism*
Glycolysis
Histidine / metabolism
Leucine / metabolism
Male
Mice
Mice, Inbred C3H
Grant Support
ID/Acronym/Agency:
1 R23 DE05393-02/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 61-90-5/Leucine; 71-00-1/Histidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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