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The distribution of vestibular efferent neurons receiving innervation of secondary vestibular afferent nerves in rats.
MedLine Citation:
PMID:  23483514     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVES/HYPOTHESIS: To explore the innervation areas of the medial vestibular nucleus (MVN) afferent neurons onto vestibular efferent neurons in the brain stem of rats. STUDY DESIGN: A morphology study in the central vestibular system. METHODS: Two neuronal tracers were used. Lectin PHA-L Conjugates (PHA-L, Invitrogen L - 11270,) was injected into the MVN as an anterograde tracer, and 5% FluoSpheres carboxylate-modified microspheres (MFS, Molecular Probe F-8793) was injected into the contralateral peripheral vestibule using as a retrograde tracer. All animals were allowed to recover for 12 days to facilitate sufficient transportation of the tracers. Then brain stems were sliced coronally on a freezing microtome and observed under a fluorescence microscope and laser confocal microscopy. RESULTS: Neurons in the MVN labeled with PHA-L exhibited green fluorescence, and their axons were distributed near the genu of the facial nerve (g7) and in the reticulation structure, as well as in the cerebellum or oculomotor-related nuclei. Neurons labeled with red fluorescence of MFS were mainly located dorsomedial and dorsolateral to g7 and in the caudal pontine reticular nucleus (PnC) bilaterally and presented different morphologies at different locations. The synaptic junctions would display color overlap (fluoresced yellow). Under three-dimensional reconstruction of the confocal laser microscopy, the synaptic junctions were visualized dorsomedial and dorsolateral to g7 bilaterally, predominantly ipsilateral to the MVN injection site. CONCLUSIONS: Morphologic evidence of the distribution of vestibular efferent neurons synapsed by afferent nerves from MVN was demonstrated. These efferent neurons constitute short closed-loop circuits with neurons in the MVN.
Authors:
Jing Wang; Fang-Lu Chi; Yuan Xin; Michael F Regner
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-3-11
Journal Detail:
Title:  The Laryngoscope     Volume:  -     ISSN:  1531-4995     ISO Abbreviation:  Laryngoscope     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-3-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607378     Medline TA:  Laryngoscope     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 The American Laryngological, Rhinological, and Otological Society, Inc.
Affiliation:
Department of Otology and Skull Base Surgery, Eye and ENT Hospital, Fudan University, Shanghai, China; Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice of P.R. China, Shanghai, China.
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