Document Detail


The distribution of serum albumin in human normal and degenerate articular cartilage.
MedLine Citation:
PMID:  1276178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for studying the distribution of a high molecular weight solute (serum albumin) between physiological saline and human articular cartilage is described. Samples of normal and fibrillated articular cartilage from both femoral condyles and femoral heads have been studied. Limited studies have also been performed where the glycosaminoglycan content of normal cartilage has been reduced by chemical or enzymic methods. With naturally occurring cartilage a wide range of partition coefficients (0.3 to less than 0.002) was obtained. The partition coefficients are very dependent upon proteoglycan concentration, with the partition coefficient decreasing with increasing fixed charge density. An attempt is made to interpret the observed partitioning in terms of the steric exclusion by the proteoglycans.
Authors:
J M Snowden; A Maroudas
Related Documents :
8900948 - High performance capillary electrophoresis method to characterize heparin and heparan s...
6523498 - Sulfated proteoglycans synthesized in myometrium of the estrogen-treated rabbit.
14558628 - Capillary electrophoretic separation of heparin oligosaccharides under conditions amena...
8241818 - Determination of methylene chloride acceptability and its "purification" for ethyleneth...
21081238 - Selectable one-dimensional or two-dimensional gas chromatography-olfactometry/mass spec...
10216988 - Simultaneous detection and quantification of acidic and basic drugs in serum toxicologi...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  428     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-09-01     Completed Date:  1976-09-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  726-40     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Binding Sites
Cartilage Diseases / metabolism*
Cartilage, Articular / metabolism*
Humans
Kinetics
Mathematics
Protein Binding
Serum Albumin / isolation & purification,  metabolism*
Chemical
Reg. No./Substance:
0/Serum Albumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protein-lipid interactions in the sialic acid incorporating system of liver microsomes.
Next Document:  The effects of proteolytic enzymes on the mechanical properties of adult human articular cartilage.