Document Detail


The disruption of circadian clockwork in differentiating cells from rat reproductive tissues as identified by in vitro real-time monitoring system.
MedLine Citation:
PMID:  17535879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The circadian clock, regulating hormonal secretion and metabolisms in accordance with the environmental light-dark cycle, resides in almost all peripheral tissues as well as in the superchiasmatic nucleus. Clock gene expression has been found to be noncyclic during spermatogenesis and the differentiation of thymocytes. However, currently little is known about how cell differentiation could affect circadian clockwork. We performed this study using the in vitro real-time oscillation monitoring system to examine the clockwork in several types of differentiating cells originated from reproductive tissues of transgenic rats (constructed with Period gene 2 (Per2) promoter-destabilized luciferase reporter gene). After treatment with dexamethasone (DXM), persistent oscillation of Per2 expression was observed in both gonadotropin-induced and pregnant ovarian luteal cells, proliferative uterine stromal cells (USCs), and nondifferentiating testicular interstitial cells, with a cyclic period of ~24 h. In contrast to these cell types, only one cycle of oscillation was sustained in granulosa cells undergoing differentiation. Additionally, Per2 oscillation was irregular in USCs undergoing decidualization induced by medroxyprogesterone acetate plus N6, 2-O-dibutyryl adenosine 3':5'-cyclic monophosphate. Furthermore, no oscillation of Per2 expression was evoked by DXM in Leydig cells and thymocytes. In conclusion, the present study characterized the oscillation of Per2 gene expression in several types of ovarian, uterine, and testicular cells, and it is strongly suggested that circadian clockwork is affected during cellular differentiation.
Authors:
Pei-Jian He; Masami Hirata; Nobuhiko Yamauchi; Seiichi Hashimoto; Masa-Aki Hattori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  193     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-30     Completed Date:  2007-08-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  413-20     Citation Subset:  IM    
Affiliation:
Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Biological Clocks / genetics*
Cell Cycle Proteins / genetics*,  metabolism
Cell Differentiation / physiology
Cells, Cultured
Computer Systems
Corpus Luteum / cytology
Female
Gene Expression Regulation*
Male
Nuclear Proteins / genetics*,  metabolism
Period Circadian Proteins
Pregnancy
Promoter Regions, Genetic
RNA, Messenger / analysis
Rats
Stromal Cells / metabolism
Testis / metabolism
Uterus / metabolism
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Nuclear Proteins; 0/Per2 protein, rat; 0/Period Circadian Proteins; 0/RNA, Messenger

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