Document Detail


A disintegrin and metalloprotease protein-12 as a novel marker for the diagnosis of ectopic pregnancy.
MedLine Citation:
PMID:  21277576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate the performance of a novel biomarker, a disintegrin and metalloprotease-12 (ADAM-12), to differentiate an ectopic pregnancy (EP) from normal intrauterine pregnancies (IUPs).
DESIGN: Case-control study.
SETTING: Three urban academic centers.
PATIENT(S): Women who were seen in the emergency department with pain or bleeding in the first trimester of pregnancy.
INTERVENTION(S): Sera from women with diagnosed EP or IUP were evaluated via proteomics and an ADAM-12 dissociation-enhanced lanthanide fluoroimmunoassay.
MAIN OUTCOME MEASURE(S): Differences between groups, area under the receiver operating curve, sensitivity, and specificity.
RESULT(S): Via a proteomics evaluation, we found a statistically significant decrease in ADAM-12 in the sera of patients with EP, which we confirmed in a larger group of 199 patients (median IUP 18.6 ng/mL versus median EP 2.5 ng/mL with good discrimination between the groups as assessed by receiver operating characteristics [area under the curve = 0.82]). At a low cut-point, the sensitivity was 70% and specificity 84%, but, at a higher cut-point optimizing sensitivity, the ADAM-12 test demonstrated a sensitivity of 97%.
CONCLUSION(S): ADAM-12 is a promising marker for the diagnosis of EP in women with symptoms in the first trimester, validating the proteomics findings. Further studies in additional patient populations and in combination with other biomarkers are needed.
Authors:
Mary E Rausch; Lynn Beer; Mary D Sammel; Peter Takacs; Karine Chung; Alka Shaunik; David Speicher; Kurt T Barnhart
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Publication Detail:
Type:  Comparative Study; Journal Article; Multicenter Study; Research Support, N.I.H., Extramural     Date:  2011-02-01
Journal Detail:
Title:  Fertility and sterility     Volume:  95     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-07     Completed Date:  2011-05-12     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1373-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
ADAM Proteins / blood*
Adult
Biological Markers / blood
Case-Control Studies
Disintegrins / blood*
Female
Humans
Matrix Metalloproteinase 12 / blood
Membrane Proteins / blood*
Pregnancy
Pregnancy Trimester, First / blood
Pregnancy, Ectopic / blood*,  diagnosis*
Proteomics / methods
Young Adult
Grant Support
ID/Acronym/Agency:
K24 HD060687/HD/NICHD NIH HHS; K24 HD060687-01A1/HD/NICHD NIH HHS; K24HD060687/HD/NICHD NIH HHS; P30 CA010815/CA/NCI NIH HHS; R01 HD036455/HD/NICHD NIH HHS; R01 HD036455-10/HD/NICHD NIH HHS; R01-HD036455/HD/NICHD NIH HHS; T32-HD007440/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Disintegrins; 0/Membrane Proteins; EC 3.4.24.-/ADAM 12 protein; EC 3.4.24.-/ADAM Proteins; EC 3.4.24.65/Matrix Metalloproteinase 12
Comments/Corrections
Comment In:
Fertil Steril. 2011 May;95(6):e21; author reply e22   [PMID:  21392752 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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