Document Detail


A discriminative analytical method for detection of CES1A1 and CES1A2/CES1A3 genetic variants.
MedLine Citation:
PMID:  22237548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human carboxylesterase 1 (hCES1), encoded by the CES1 gene, is the predominant hepatic hydrolase responsible for the metabolism of many therapeutic agents, toxins, and endogenous substances. Genetic variants of CES1 can affect hCES1 function and expression and ultimately influence clinical response to drugs serving as hCES1 substrates. The CES1 gene consists of three isoforms including the functional CES1A1 and CES1A2 genes and the nonfunctional pseudogene CES1A3. Natural variants of these isoforms exert differing impacts on hCES1 function. However, the existing CES1 genotyping methods are incapable of determining whether these variants belong to CES1A1, CES1A2, or CES1A3 because of the high similarity among these three genes, as a consequence they are unable to discriminate between heterozygotes and homozygotes. We report the development of a novel long-range PCR-based, discriminative genotyping assay capable of specifically detecting the variants among CES1A1, CES1A2, and CES1A3 genes. The comparison of the genotyping results between this novel assay and those previously reported methods highlighted the necessity of applying the discriminative genotyping assay in pharmacogenetic studies involving CES1 gene.
Authors:
Hao-Jie Zhu; Bryan Brinda; Tanya E Froehlich; John S Markowitz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Pharmacogenetics and genomics     Volume:  22     ISSN:  1744-6880     ISO Abbreviation:  Pharmacogenet. Genomics     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-08     Completed Date:  2012-08-01     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  101231005     Medline TA:  Pharmacogenet Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  215-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carboxylic Ester Hydrolases / genetics*
Genotyping Techniques*
Heterozygote
Homozygote
Humans
Polymerase Chain Reaction / methods*
Protein Isoforms / genetics*
Grant Support
ID/Acronym/Agency:
K23 MH083881/MH/NIMH NIH HHS; K23 MH083881-01A1/MH/NIMH NIH HHS; K23MH083881/MH/NIMH NIH HHS; UL1 TR000064/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Protein Isoforms; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.1/CES1 protein, human
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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