Document Detail


A directory of human germ-line V kappa segments reveals a strong bias in their usage.
MedLine Citation:
PMID:  8149953     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
From the genomic DNA of a single individual, we have amplified, cloned and sequenced 37 human germ-line V kappa segments. Four of these segments were new. We then compiled a comprehensive directory of all germ-line V kappa segments and identified 50 different sequences with open reading frames. Comparison with 236 rearranged sequences revealed that no more than 24 of these germ-line sequences could be assigned rearranged counterparts, that some of these were rarely used, and that only about 11 sequences are used frequently. This suggests that the expressed V kappa repertoire is mainly derived from a limited number of segments. Most surprisingly, the J kappa-distal region of the locus appears to be rarely used: we could unambiguously assign 162 rearranged sequences to V kappa segments of the J kappa-proximal region, but only 5 to segments of the J kappa-distal region.
Authors:
J P Cox; I M Tomlinson; G Winter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  24     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-05-09     Completed Date:  1994-05-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  827-36     Citation Subset:  IM    
Affiliation:
MRC Centre for Protein Engineering, Cambridge.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Z27498;  Z27499;  Z27500;  Z27501
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Gene Rearrangement
Genes, Immunoglobulin*
Humans
Immunoglobulin Variable Region / chemistry,  genetics*
Immunoglobulin kappa-Chains / chemistry,  genetics*
Molecular Sequence Data
Chemical
Reg. No./Substance:
0/Immunoglobulin Variable Region; 0/Immunoglobulin kappa-Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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