Document Detail

The direct and interactive effects of phosphodiesterase inhibition and beta-adrenergic stimulation on myocyte contractile function after hypothermic cardioplegic arrest.
MedLine Citation:
PMID:  7486079     Owner:  NLM     Status:  MEDLINE    
The direct and interactive effects of phosphodiesterase inhibition (PDEI) and beta-adrenergic receptor (beta AR) stimulation on isolated myocyte contractile function were examined after hypothermic, hyperkalemic, cardioplegic arrest (HHCA) and under normothermic conditions. Left ventricular (LV) myocytes were isolated from porcine hearts and myocyte contractile function was measured under normothermic conditions (37 degrees C in standard media) and after HHCA (2 h at 4 degrees C in Ringer's solution with 24 mEq KCl) with subsequent rewarming. Myocytes were then randomly assigned to treatment with the beta AR agonist isoproterenol (25 nM), the phosphodiesterase inhibitor amrinone (50 microM), or a combination of these compounds and contractile function measurements repeated. Baseline myocyte contractile function was reduced by 32% after HHCA. Isoproternol alone increased myocyte contractile function more than 100% under both normothermic conditions and after HHCA, whereas amrinone alone significantly (60%) improved myocyte contractile function only after HHCA. Amrinone preincubation followed by isoproterenol improved contractile function after HHCA to a greater extent than all other treatment protocols. In contrast, combination treatment under normothermic conditions did not augment myocyte contractile function relative to isoproterenol alone. These findings suggest that amrinone has differential effects on contractile processes. Moreover, the marked improvement of contractile function after HHCA with PDEI pretreatment followed by beta AR stimulation may have implications in treatment strategies for improving myocardial function after cardiopulmonary bypass and provide insight into contractile dysfunction after HHCA.
B H Dorman; M J Cavallo; R C Roy; F G Spinale
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Anesthesia and analgesia     Volume:  81     ISSN:  0003-2999     ISO Abbreviation:  Anesth. Analg.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-19     Completed Date:  1995-12-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1310650     Medline TA:  Anesth Analg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  925-31     Citation Subset:  AIM; IM    
Department of Anesthesiology, Medical University of South Carolina, Charleston 29425-2207, USA.
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MeSH Terms
Adrenergic beta-Agonists / administration & dosage*
Amrinone / administration & dosage*
Cold Temperature*
Drug Interactions
Heart Arrest, Induced*
Isoproterenol / administration & dosage*
Myocardial Contraction / drug effects*
Phosphodiesterase Inhibitors / administration & dosage*
Reg. No./Substance:
0/Adrenergic beta-Agonists; 0/Phosphodiesterase Inhibitors; 60719-84-8/Amrinone; 7683-59-2/Isoproterenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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