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A direct competitive enzyme-linked immunosorbent assay for rapid detection of anilofos residues in agricultural products and environmental samples.
MedLine Citation:
PMID:  23030434     Owner:  NLM     Status:  In-Data-Review    
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed to measure anilofos levels in agricultural and environmental samples. The ELISA was developed using rabbit polyclonal antibodies against a hapten-protein conjugate of anilofos-bovine serum albumin. The limit of detection was 0.1 μg L(-1), and there was no cross-reactivity with other related pesticides or structurally similar compounds. The matrix effects of rice (aromatic rice, white rice, brown rice), corn, barley, wheat and soil were measured and removed by extraction and dilution with phosphate buffered saline with 0.05% Tween-20. For water samples (tap water and river water), the matrix effects were also removed by dilution with phosphate buffered saline with Tween-20. The detection limits for anilofos in authentic samples (aromatic rice, white rice, brown rice, corn, barley, wheat, soil, tap water and river water) were 2, 2, 2, 3, 2, 2, and 2 μg kg(-1), and 0.5 and 1 μg L(-1), respectively . The anilofos recovery ranged from 81.0-116.0% with a coefficient of variation of 1.7-9.0%. The method was validated using GC, and the results showed good correlation with the dc-ELISA data (r(2) = 0.9795). Forty-two cereal samples were randomly collected from different supermarkets and analyzed using the developed dc-ELISA. No anilofos was found in these products. The developed immunoassay is suitable for rapid quantitation of anilofos residues.
Yan Zhang; Ai H Gao; Bing Liu; Wei Sheng; Chao Tan; Meng Yuan; Shuo Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes     Volume:  48     ISSN:  1532-4109     ISO Abbreviation:  J Environ Sci Health B     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7607167     Medline TA:  J Environ Sci Health B     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
a Key Laboratory of Food Nutrition and Safety, Ministry of Education of China , Tianjin University of Science and Technology , Tianjin , China.
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