| A direct comparison of the performance of ground, beaded and silica-grafted MIPs in HPLC and turbulent flow chromatography applications. | |
| | |
MedLine Citation:
|
PMID: 15556354 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Spherical molecularly imprinted polymers (MIPs) specific to the beta-blocker propranolol have been synthesised using two different approaches and compared to traditional ground monolithic MIPs in HPLC and TFC applications. TFC is a LC technique used for rapid extraction of compounds directly from complex matrices. It can be easily coupled to HPLC and MS for automation of an extraction/analysis procedure. Spherical MIP beads were produced using a suspension polymerisation technique and silica/MIP composite beads by grafting MIP to spherical silica particles using a surface-bound initiator species. Synthesis of both beaded and silica-grafted MIPs was more practical than using the traditional grinding method and yields of spherical particles of the required size between 80 and 100% were routinely achieved. Under HPLC conditions, beaded and ground MIP materials showed a degree of chiral separation for all of the nine beta-blockers tested. The beaded MIP, however, showed much better flow properties and peak shape than the ground material. Silica-grafted MIP showed some separation in five of the drugs and a large improvement in peak shape and analysis times compared with both ground and beaded MIPs. The materials prepared were also used in extraction columns for Turbulent Flow Chromatography (TFC). Although no imprinting effect was observed under typical TFC conditions, beaded polymer materials showed promise for use as TFC extraction columns due to the good flow properties and clean extracts obtained. |
| | |
Authors:
|
Robert E Fairhurst; Christophe Chassaing; Richard F Venn; Andrew G Mayes |
Related Documents
:
|
16863774 - Sequential bypass grafting on the beating heart: blood flow characteristics. 927394 - Biological evaluation of biomaterials for cardiovascular applications: some current res... 6524224 - Experimental microvascular sleeve anastomoses. 19684884 - Extensive deendothelialization and thrombogenicity in routinely prepared vein grafts fo... 6696024 - Formed visual hallucinations after metrizamide myelography. 3109894 - Effects of a heat and moisture exchanger on carbon dioxide equilibrium during mechanica... |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biosensors & bioelectronics Volume: 20 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2004 Dec |
Date Detail:
|
Created Date: 2004-11-23 Completed Date: 2005-06-03 Revised Date: 2009-07-14 |
Medline Journal Info:
|
Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
|
Languages: eng Pagination: 1098-105 Citation Subset: IM |
Affiliation:
|
Pfizer Global R&D Ramsgate Road, Sandwich, Kent, UK. robert.fairhurst@uea.ac.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenergic beta-Antagonists
/
chemistry*,
isolation & purification* Chromatography, High Pressure Liquid / instrumentation, methods* Chromatography, Liquid / instrumentation, methods* Microfluidics / instrumentation, methods* Microspheres Particle Size Propranolol / chemistry*, isolation & purification* Silicon Dioxide / chemistry* Surface Properties |
| Chemical | |
Reg. No./Substance:
|
0/Adrenergic beta-Antagonists; 525-66-6/Propranolol; 7631-86-9/Silicon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Imprinted polymer layer for recognizing double-stranded DNA.
Next Document: Electronic transduction in model enzyme sensors assisted by a photoisomerizable azo-polymer.