Document Detail


A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli.
MedLine Citation:
PMID:  8987689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Different approaches to increasing carbon commitment to aromatic amino acid biosynthesis were compared in isogenic strains of Escherichia coli. In a strain having a wild-type PEP:glucose phosphotransferase (PTS) system, inactivation of the genes encoding pyruvate kinase (pykA and pykF) resulted in a 3.4 fold increase in carbon flow to aromatic biosynthesis. In a strain already having increased carbon flow to aromatics by virtue of overexpression of the tktA gene (encoding transketolase), the pykA and/or pykf mutations had no effect. A PTS- glucose+ mutant showed a 1.6-fold increase in carbon flow to aromatics compared to the PTS+ control strain. In the PTS- glucose+ host background, overexpression of tktA caused a further 3.7-fold increase in carbon flow, while inactivation of pykA and pykF caused a 5.8-fold increase. When all of the variables tested (PTS-glucose+, pykA, pykF, and overexpressed tktA) were combined in a single strain, a 19.9-fold increase in carbon commitment to aromatic biosynthesis was achieved.
Authors:
G Gosset; J Yong-Xiao; A Berry
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of industrial microbiology     Volume:  17     ISSN:  0169-4146     ISO Abbreviation:  J. Ind. Microbiol.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1997-01-30     Completed Date:  1997-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8610887     Medline TA:  J Ind Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  47-52     Citation Subset:  B    
Affiliation:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / biosynthesis*
Carbon / metabolism*
Escherichia coli / growth & development,  metabolism*
Genes, Bacterial / genetics
Mutation
Phosphoenolpyruvate Sugar Phosphotransferase System / physiology
Pyruvate Kinase / genetics
Sugar Acids / metabolism
Transketolase / genetics
Chemical
Reg. No./Substance:
0/5-dehydro-3-deoxy-D-arabino-heptulosonic acid-7-phosphate; 0/Amino Acids; 0/Sugar Acids; 7440-44-0/Carbon; EC 2.2.1.1/Transketolase; EC 2.7.1.-/Phosphoenolpyruvate Sugar Phosphotransferase System; EC 2.7.1.40/Pyruvate Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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