Document Detail


A direct assay for evaluation of polyethylene glycol in enzyme adducts used as drugs or biocatalysts.
MedLine Citation:
PMID:  2400529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A specific and direct method for the evaluation of monomethoxypolyethylene glycol (MPEG) in enzyme adducts has been developed. The method is based on extensive modification by trinitrobenzensulfonic acid (TNBS) of the MPEG-enzyme, to form an acid-stable TNBS adduct with the available amino groups of lysine and alpha-amino acid. The MPEG-modified trinitrophenylated enzyme was hydrolyzed in 6N HCl and amino acid composition evaluated by a standard AA analyzer in comparison with the analysis of a MPEG enzyme sample. The number of bound MPEG polymers was calculated from the difference in amino acid composition of the two samples.
Authors:
O Schiavon; L Sartore; P Caliceti; F M Veronese
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Farmaco (Società chimica italiana : 1989)     Volume:  45     ISSN:  0014-827X     ISO Abbreviation:  Farmaco     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-10-26     Completed Date:  1990-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8912641     Medline TA:  Farmaco     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  791-5     Citation Subset:  IM    
Affiliation:
Dipartimento di Scienze Farmaceutiche, Università Degli Studi di Padova, Italy.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Catalysis
Enzyme Activation
Enzymes / analysis*,  diagnostic use
Polyethylene Glycols / analysis*
Ribonucleases / analysis
Superoxide Dismutase / analysis
Trinitrobenzenesulfonic Acid
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Enzymes; 0/Polyethylene Glycols; 2508-19-2/Trinitrobenzenesulfonic Acid; EC 1.15.1.1/Superoxide Dismutase; EC 3.1.-/Ribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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