| The dimeric transmembrane domain of prolyl dipeptidase DPP-IV contributes to its quaternary structure and enzymatic activities. | |
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MedLine Citation:
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PMID: 20572019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dipeptidyl peptidase IV (DPP-IV) is a drug target in the treatment of human type II diabetes. It is a type II membrane protein with a single transmembrane domain (TMD) anchoring the extracellular catalytic domain to the membrane. DPP-IV is active as a dimer, with two dimer interacting surfaces located extracellularly. In this study, we demonstrate that the TM of DPP-IV promotes DPP-IV dimerization and rescues monomeric DPP-IV mutants into partial dimers, which is specific and irreplaceable by TMs of other type II membrane proteins. By bioluminescence resonance energy transfer (BRET) and peptide electrophoresis, we found that the TM domain of DPP-IV is dimerized in mammalian cells and in vitro. The TM dimer interaction is very stable, based on our results with TM site-directed mutagenesis. None of the mutations, including the introduction of two prolines, resulted in their complete disruption to monomers. However, these TM proline mutations result in a significant reduction of DPP-IV enzymatic activity, comparable to what is found with mutations near the active site. A systematic analysis of TM structures deposited in the Protein Data Bank showed that prolines in the TM generally produce much bigger kinking angles than occur in nonproline-containing TMs. Thus, the proline-dependent reduction in enzyme activity may result from propagated conformational changes from the TM to the extracellular active site. Our results demonstrate that TM dimerization and conformation contribute significantly to the structure and activity of DPP-IV. Optimal enzymatic activity of DPP-IV requires an optimal interaction of all three dimer interfaces, including its TM. |
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Authors:
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Kuei-Min Chung; Jai-Hong Cheng; Ching-Shu Suen; Chih-Hsiang Huang; Cheng-Han Tsai; Li-Hao Huang; Yi-Rong Chen; Andrew H-J Wang; Weir-Torn Jiaang; Ming-Jing Hwang; Xin Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protein science : a publication of the Protein Society Volume: 19 ISSN: 1469-896X ISO Abbreviation: Protein Sci. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-11-05 Completed Date: 2011-03-11 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 9211750 Medline TA: Protein Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1627-38 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 The Protein Society. |
Affiliation:
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Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Zhu Nan town, Miaoli County, Taiwan, Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Dipeptidyl Peptidase 4 / chemistry*, genetics, metabolism* Humans Models, Molecular Molecular Sequence Data Mutation Protein Multimerization Protein Structure, Quaternary Protein Structure, Tertiary Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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EC 3.4.14.5/Dipeptidyl Peptidase 4 |
| Comments/Corrections | |
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