Document Detail


The differentiation and isolation of mouse embryonic stem cells toward hepatocytes using galactose-carrying substrata.
MedLine Citation:
PMID:  22118818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple culture system to achieve the differentiation of embryonic stem (ES) cells toward hepatocytes with high efficiency is crucial in providing a cell source for the medical application. In this study, we report the effect of a matrix-dependent enrichment of ES cell-derived hepatocytes using immobilized poly(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide) (PVLA) with E-cadherin-IgG Fc (E-cad-Fc) as a galactose-carrying substratum. PVLA and E-cad-Fc were confirmed to be stably co-adsorbed onto polystyrene surface by quartz crystal microbalance (QCM). We showed that the E-cad-Fc/PVLA hybrid substratum was efficient in culturing primary hepatocytes and maintaining liver functions, on which the undifferentiated ES cells also maintained high proliferative capability. Furthermore, ES cell-derived hepatocytes on this hybrid matrix expressed elevated level of liver specific genes and functions together with early expression of definitive hepatocyte marker, asialoglycoprotein receptor (ASGPR). Finally, we isolated a high percentage of cells (about 60%) with ASGPR expression after re-seeding onto PVLA-coated surface, and observed the elimination of the poorly differentiated cells (Gata6(+) and Sox17(+)) and the ones toward another cell lineage (brachyury(+) and Pdx1(+)). The system uses a glycopolymer as an extracellular substratum for isolation and enrichment of ES cell-derived hepatocytes with adequate homogeneity and functionality.
Authors:
Qingyuan Meng; Amranul Haque; Bayar Hexig; Toshihiro Akaike
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-26
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2011-12-13     Completed Date:  2012-04-02     Revised Date:  2012-04-23    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1414-27     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adsorption / drug effects
Animals
Asialoglycoprotein Receptor / metabolism
Cadherins / metabolism
Cell Differentiation / drug effects*
Cell Separation / methods*
Cells, Cultured
Disaccharides / pharmacology*
Embryonic Stem Cells / cytology*,  drug effects*,  metabolism
Endoderm / cytology,  drug effects,  metabolism
Galactose / pharmacology*
Hepatocytes / cytology*,  drug effects,  metabolism
Immobilized Proteins / metabolism
Mice
Phenotype
Polystyrenes / pharmacology
Receptors, Fc / metabolism
Surface Properties / drug effects
Vinyl Compounds / pharmacology*
Chemical
Reg. No./Substance:
0/Asialoglycoprotein Receptor; 0/Cadherins; 0/Disaccharides; 0/Immobilized Proteins; 0/Polystyrenes; 0/Receptors, Fc; 0/Vinyl Compounds; 0/poly-(N-p-vinylbenzyl-O-galactopyranosyl-(1-4)-gluconamide); 26566-61-0/Galactose
Comments/Corrections
Erratum In:
Biomaterials. 2012 May;33(15):4010-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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