Document Detail


The differentiation-inducing effect of conditioned media obtained from dental pulp cells.
MedLine Citation:
PMID:  19426909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Conditioned media (CM) from human dental pulp cells (HDPC) was investigated for its effects on the proliferation and differentiation of HDPC and MG63 cells. STUDY DESIGN: CM prepared from the primary culture of HDPC was used for the culture of HDPC and MG63. Cell growth, alkaline phosphatase (ALPase) activity, mRNA expression of differentiation-related genes, and mineralization of the HDPC and MG63 cells in the media containing CM were assessed. RESULTS: HDPC CM increased the cell growth of HDPC but decreased MG63 cell growth. ALPase activity and the mineralization of both HDPC and MG63 were enhanced by HDPC CM. The CM also up-regulated the expressions of DSPP, DMP-1, and OCN mRNA in HDPC. Pretreatment of HDPC CM with a neutralizing antibody against TGF-beta completely eliminated the effect of CM on ALPase activity in HDPC. CONCLUSION: HDPC was able to secrete odontogenic differentiation-inducing factors, in which TGF-beta seems to a key element of the CM effects.
Authors:
Na Ryoung Kim; Dong Hee Lee; Sug-Joon Ahn; In-Seop Lee; Hyeong-Cheol Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics     Volume:  107     ISSN:  1528-395X     ISO Abbreviation:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-11     Completed Date:  2009-08-03     Revised Date:  2010-02-05    
Medline Journal Info:
Nlm Unique ID:  9508562     Medline TA:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e54-9     Citation Subset:  D; IM    
Affiliation:
Department of Dental Biomaterials Science, School of Dentistry, Seoul National University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Alkaline Phosphatase / biosynthesis
Calcification, Physiologic / drug effects
Cell Differentiation / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Cells, Cultured
Child
Culture Media, Conditioned / pharmacology*
Dental Pulp / cytology,  physiology*
Dentin, Secondary / growth & development,  secretion*
Extracellular Matrix Proteins / biosynthesis
Gene Expression Regulation, Developmental
Humans
Odontoblasts / metabolism*
Osteocalcin / biosynthesis
Phosphoproteins / biosynthesis
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Transforming Growth Factor beta / antagonists & inhibitors,  physiology*
Up-Regulation
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/DMP1 protein, human; 0/Extracellular Matrix Proteins; 0/Phosphoproteins; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 0/dentin sialophosphoprotein; 104982-03-8/Osteocalcin; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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