Document Detail


A differentiation-defective concanavalin-A-resistant variant of a pluripotent embryonal carcinoma cell line.
MedLine Citation:
PMID:  3428508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A concanavalin-A(Con A)-resistant variant of the pluripotent mouse embryonal carcinoma cell line, PSA1-NG2, was isolated. This variant, designated NG2-2.16, fails to exhibit the extensive spontaneous differentiation displayed by PSA1-NG2 in colonies in vitro and in tumours in vivo. The molecular nature of the defect in NG2-2.16 cells was not revealed by quantitative studies of the binding, uptake and metabolism of tritiated Con A, or by Western blotting of membrane and whole cell homogenates, thus indicating the defect to be the result of a more subtle molecular alteration. Statistical evidence suggests that the same mutation is responsible for both the Con A resistance and the lack of spontaneous differentiation. NG2-2.16 cells were induced to differentiate by exposure to retinoic acid, suggesting that the mutation affects the regulation of differentiation rather than the potential for differentiation.
Authors:
P Koopman; C Thomas; K J Fowler; H J McArdle; R G Cotton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  34     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1987  
Date Detail:
Created Date:  1988-02-29     Completed Date:  1988-02-29     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  216-21     Citation Subset:  IM    
Affiliation:
Murdoch Institute for Research into Birth Defects, Royal Children's Hospital, Parkville, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / drug effects
Cell Line
Concanavalin A / metabolism,  pharmacokinetics,  pharmacology*
Drug Resistance
Mice
Mutation
Teratoma / genetics,  metabolism,  pathology*
Tretinoin / pharmacology
Chemical
Reg. No./Substance:
11028-71-0/Concanavalin A; 302-79-4/Tretinoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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