Document Detail


The differential effect of genetic variation on soluble CD14 levels in human plasma and milk.
MedLine Citation:
PMID:  15373760     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PROBLEM: The protein CD14 is a pattern recognition receptor for bacterial lipopolysaccharide (LPS). Whether genetic variation has the same influence on soluble CD14 (sCD14) levels in human plasma and milk remains to be determined. METHOD OF STUDY: We measured sCD14 levels in plasma during pregnancy (n = 196) and in milk in the postpartum (n = 152) for women genotyped for the single nucleotide polymorphisms (SNPs) at positions -1619, -550, and -159 from the transcription start site of the CD14 gene. RESULTS: Plasma- and milk-sCD14 levels differed significantly both by CD14/-1619 and CD14/-550 genotypes and by haplotypes. Most interestingly, sCD14 levels were regulated differentially by the same genetic variants in plasma and milk, with the CD14/-550T allele and the corresponding are ATC haplotype associated with high sCD14 in milk but low sCD14 in plasma. A correlation between sCD14 levels in plasma and milk was absent (r = 0.091, P = NS). CONCLUSIONS: Our findings suggest the existence of cell-specific regulation mechanisms of CD14 gene expression.
Authors:
Stefano Guerra; I Carla Lohman; Tricia D LeVan; Anne L Wright; Fernando D Martinez; Marilyn Halonen
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  52     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-17     Completed Date:  2005-03-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  204-11     Citation Subset:  IM    
Affiliation:
Arizona Respiratory Center, College of Medicine, University of Arizona, Tuscon, AZ 85724-5030, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aging
Antigens, CD14 / blood*,  genetics*,  metabolism
Breast Feeding
Ethnic Groups
Female
Gene Expression Regulation
Genotype
Humans
Milk, Human / chemistry*
Parity
Polymorphism, Single Nucleotide / genetics*
Pregnancy
Solubility
Grant Support
ID/Acronym/Agency:
AI42268/AI/NIAID NIH HHS; AI44697/AI/NIAID NIH HHS; HL61892/HL/NHLBI NIH HHS; HL67672/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD14

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