| A device to measure oxygen consumption during the hypothermic perfusion of the liver. | |
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MedLine Citation:
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PMID: 19946656 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work deals with the construction and performance of a device designed to measure the oxygen consumption by the liver during hypothermic perfusion in the rat model. Due to its simple design and the utilization of standard materials, it could serve to determine the role of oxygenation during hypothermic perfusion of the liver. The system consists of a reservoir containing the preservation solution, a peristaltic pump and an internal oxygenator made of silicone tube. A five ports manifold connects the circulation to the liver (inflow), to a hydrostatic manometer and to two sample ports; the liver outflow and temperature sensor or gas calibration. Finally the exit port connects the circulation fluid with an oxygen electrode. The preservation solution is pumped through the liver at a constant pressure (77 i 15 mm H2O) and a perfusion flow of 0.39 - 0.49 mL per min per g liver. To test the system, two to four hours perfusion experiments were performed, at temperatures of 5 and 10 degree C. Two preservation solutions were evaluated: Custodiol and Bes-Gluconate-Sucrose. The solubility of oxygen in the preservation solutions was determined, and the oxygen consumption by preserved rat livers was measured. |
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Authors:
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Joaqu?n V Rodriguez; Mar?a Belen Federico; Mar?a Dolores Pizarro; Edgardo E Guibert; Alejandra B Quintana; Angel L Scandizzi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cryo letters Volume: 30 ISSN: 0143-2044 ISO Abbreviation: Cryo Letters Publication Date: 2009 Sep-Oct |
Date Detail:
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Created Date: 2009-11-30 Completed Date: 2010-03-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9891832 Medline TA: Cryo Letters Country: England |
Other Details:
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Languages: eng Pagination: 335-46 Citation Subset: IM |
Affiliation:
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Farmacolog?a, Departamento Ciencias, Fisiologicas, Facultad de Ciencias, Bioquimicas y Farmaceuticas, Rosario, Argentina. jrodrig@fbioyf.unr.edu.ar |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cryoprotective Agents / pharmacology Electrodes Equipment and Supplies* Gluconates / pharmacology Glucose / pharmacology Hypothermia / pathology, physiopathology* Infusion Pumps Liver / drug effects, pathology, physiopathology* Male Mannitol / pharmacology Manometry Oxygen Consumption / physiology* Potassium Chloride / pharmacology Procaine / pharmacology Rats Rats, Wistar Regression Analysis Sucrose / pharmacology Temperature |
| Chemical | |
Reg. No./Substance:
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0/Bretschneider cardioplegic solution; 0/Cryoprotective Agents; 0/Gluconates; 50-99-7/Glucose; 526-95-4/gluconic acid; 57-50-1/Sucrose; 59-46-1/Procaine; 69-65-8/Mannitol; 7447-40-7/Potassium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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