Document Detail


A device for the liberation and determination of 14CO2.
MedLine Citation:
PMID:  6414334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple closed system for the serial determination of 14CO2 in small volumes of fluid samples, is described. The device consists of commercially available scintillation vials and silicone tube seals. 14CO2 is selectively liberated by citric acid and absorbed in a scintillation vial by Hyamine. Experiments on the effect of dichloroacetate on pyruvate dehydrogenase activity in rat hindlimbs perfused with [1-14C]pyruvate demonstrate the applicability of the method.
Authors:
P Schadewaldt; M E Förster; U Münch; W Staib
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  132     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1983 Jul 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  400-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide / analysis*
Carbon Radioisotopes
Citrates
Citric Acid
Dichloroacetate / administration & dosage
Hindlimb
Kinetics
Perfusion
Pyruvate Dehydrogenase Complex / metabolism
Rats
Rats, Inbred Strains
Scintillation Counting / instrumentation*
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Citrates; 0/Pyruvate Dehydrogenase Complex; 124-38-9/Carbon Dioxide; 13425-80-4/Dichloroacetate; 77-92-9/Citric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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