| A device for aeration and mixing of cell and organelle suspensions during nuclear magnetic resonance studies. | |
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MedLine Citation:
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PMID: 2690676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A device is described which maintains homogeneous aerobic or anaerobic cell and organelle suspensions within an NMR sample tube. Line broadening due to magnetic field inhomogeneity is reduced by elimination of gas bubbles from the area of the probe receiver coils. The linewidth of the extracellular orthophosphate resonance of a yeast suspension in 31P NMR was 0.21 ppm compared with 0.4-0.7 ppm in conventionally aerated suspensions. Recirculation of the sample results in complete mixing within 90 s of addition of aliquots of acid or alkali. The maximum rate of oxygen transfer from the gaseous to the liquid phase was approximately 600 microM min-1 when aerated with 95% oxygen/5% carbon dioxide. A 60% wet weight suspension of yeast cells was recirculated for 20 h without settling of cells occurring. A method for estimating oxygen transfer rate is described. |
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Authors:
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R M Bourne |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 182 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1989 Oct |
Date Detail:
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Created Date: 1990-02-02 Completed Date: 1990-02-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 151-6 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Queensland, St. Lucia, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Candida
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cytology Cytological Techniques / instrumentation* Magnetic Resonance Spectroscopy / instrumentation*, methods Organelles Phosphorus Isotopes |
| Chemical | |
Reg. No./Substance:
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0/Phosphorus Isotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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