| The development of a sensitive and specific enzyme immunoassay for FK480, a novel cholecystokinin type-A receptor antagonist, in human plasma. | |
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MedLine Citation:
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PMID: 9800657 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive and specific enzyme immunoassay for FK480, a novel cholecystokinin type-A (CCK-A) receptor antagonist, was developed to study the pharmacokinetics of the drug at low-dose administration using a specific monoclonal antibody. The high performance liquid chromatography (HPLC) method had been used for studying toxicokinetics, but its determination limit (2.5 ng ml-1) was too high for use in clinical studies. Subsequently we developed an enzyme immunoassay (EIA) using rabbit anti-FK480 serum (polyclonal antibody). It had higher sensitivity (0.1 ng ml-1) when 0.5 ml of plasma was used but its specificity was low because of the cross-reactivity of the metabolites of FK480. Therefore we produced several monoclonal antibodies for FK480 by cell fusion, and selected the antibody which was least cross-reactive for the isolated metabolites of FK480. Finally we developed a sensitive and specific EIA using this monoclonal antibody. The lower limit of quantification of this method was 0.2 ng ml-1 when 0.2 ml of human plasma was used. The coefficient of variation over the calibration range (0.2-10 ng ml-1) was less than 15%. We used this method for clinical studies, and it showed a good correlation to the HPLC method when plasma concentration was 2.5 ng ml-1 or more. |
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Authors:
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K Nozaki; S Suzuki; F Maeda; S Takagaki; A Suzuki; T Hata |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 17 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1999-01-13 Completed Date: 1999-01-13 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1381-92 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal Benzodiazepinones / blood*, immunology, metabolism, pharmacokinetics Chromatography, High Pressure Liquid Cross Reactions Dogs Female Hormone Antagonists / blood*, immunology, metabolism, pharmacokinetics Humans Immunoenzyme Techniques Indoles / blood*, immunology, metabolism, pharmacokinetics Male Mice Mice, Inbred BALB C Rabbits Rats Rats, Sprague-Dawley Receptors, Cholecystokinin / antagonists & inhibitors* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Benzodiazepinones; 0/Hormone Antagonists; 0/Indoles; 0/Receptors, Cholecystokinin; 150408-73-4/FR 120480 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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