Document Detail


The development of monoclonal antibodies against salmon (Oncorhynchus kisutch and O. keta) pituitary hormones and their immunohistochemical identification.
MedLine Citation:
PMID:  2272459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monoclonal antibodies (MCAs) directed against several salmon pituitary hormones were generated by the fusion of myeloma cells with spleen cells from mice that had been immunized with either chum salmon (Oncorhynchus keta) growth hormone (GH) or prolactin (PRL), or one of two purified protein preparations from coho salmon (O. kisutch) pituitaries. Hybridoma were cloned by limiting dilution and screened for MCA production using immunohistochemical procedures. MCAs were generated that bound specifically to GH, PRL, or gonadotropic cells. MCAs were generated that bound to either fine granular material or large globular inclusions in the cytoplasm of the "classical" strongly PAS-positive globular gonadotropic cell type found in mature fish. This suggests that these MCAs are directed against gonadotropin II (GTH II). A MCA was also generated that bound both granular and globular material in the globular gonadotrops and granular material in the weakly PAS-positive vesicular gonadotrops in pituitaries from mature fish and to a cell type in immature rainbow trout pituitaries which is tentatively identified as the gonadotropin I (GTH I) cell type. This MCA did not bind to thyrotrops in immature rainbow trout pituitaries.
Authors:
K J Farbridge; G McDonald-Jones; C L McLean; P J Lowry; R J Etches; J F Leatherland
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  79     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1991-02-28     Completed Date:  1991-02-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  361-74     Citation Subset:  IM    
Affiliation:
Department of Zoology, University of Guelph, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / biosynthesis*
Hybridomas / immunology
Immunohistochemistry
Male
Mice
Mice, Inbred BALB C
Pituitary Gland / metabolism
Pituitary Hormones / immunology*
Prolactin / immunology
Salmon / metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Pituitary Hormones; 9002-62-4/Prolactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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