| The development of larger cells that spontaneously escape senescence--a step during the immortalization of a human cancer cell line. | |
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MedLine Citation:
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PMID: 15090264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There are few information concerning the changes associated with the transition interval when slow growing, primary explanted human cancer cells are displaced by new selected faster growing cells and became an immortal cell line. In a previous paper (J. Cell. Mol. Med., 5: 49-59, 2001) we described the TV cell line derived from a laryngeal tumor which harbors human papillomavirus (HPV) gene sequences throughout more than sixty in vitro passages. In this paper we analyze the modifications observed during the crisis interval when significant amount of cells senesce but occasional cells acquire some mutations that make them immortal. Confocal microscopy analysis revealed the heterogeneity of the cells in terms of their size and nucleus/cell ratio. Proliferation capacity was assessed by flow cytometry analyzing DNA content and expression of transferrin receptor (CD71). We discussed the possibility that HPV genome sequences alleviate a proliferation block during the crisis growth arrest of human larynx carcinoma cell line and the possibility that the cells monitor their size and growth by measuring the levels of some protein whose synthesis is coupled to cell development. |
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Authors:
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Carmen C Diaconu; Coralia Bleotu; Mihaela Chivu; Irina Alexiu; Daniela Petrusca; Gabriela Anton; Ruxandra Achim; Simona M Ruta; C Cernescu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular and molecular medicine Volume: 8 ISSN: 1582-1838 ISO Abbreviation: J. Cell. Mol. Med. Publication Date: 2004 Jan-Mar |
Date Detail:
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Created Date: 2004-04-19 Completed Date: 2004-07-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101083777 Medline TA: J Cell Mol Med Country: Romania |
Other Details:
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Languages: eng Pagination: 93-101 Citation Subset: IM |
Affiliation:
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St. S. Nicolau Institute of Virology, Bucharest, Romania. ccdiaconu@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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biosynthesis Antigens, Differentiation, B-Lymphocyte / biosynthesis Carcinoma / pathology Cell Aging Cell Culture Techniques / methods* Cell Division Cell Line* Cell Line, Tumor Cell Nucleus / metabolism Cells, Cultured DNA / chemistry Flow Cytometry Genome, Viral Humans Laryngeal Neoplasms / pathology Microscopy, Confocal Mutation Papillomaviridae / genetics Ploidies Polymerase Chain Reaction Receptors, Transferrin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Differentiation, B-Lymphocyte; 0/CD71 antigen; 0/Receptors, Transferrin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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