Document Detail

The development and characterisation of a SV40 T-antigen positive cell line of human hepatic origin.
MedLine Citation:
PMID:  9128863     Owner:  NLM     Status:  MEDLINE    
COS7 cells and other cell lines expressing the SV40 large T-antigen, have been of great benefit in transfection studies using transient expression vectors which contain the SV40 origin of replication, as these cells allow plasmid replication to a high copy number. As no T-antigen expressing cell line derived from a well characterised hepatocyte-like continuous cell line currently exists, the establishment of such a cell line for studies which require expression of hepatocyte-specific factors would be extremely useful. A HepG2-derived stable cell line (THT1) was therefore developed which demonstrates a high level of transfection efficiency whilst retaining hepatocyte-like features, such as the production of hepatitis B virus. The THT1 cell line displayed chromosomal integration of the SV40 T-antigen gene and nuclear expression of the antigen. The cell line also maintained the general morphological features of its parent cell line, and showed an increased rate of cell growth.
T J Harvey; T B Macnaughton; E J Gowans
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virological methods     Volume:  65     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-06-23     Completed Date:  1997-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  67-74     Citation Subset:  IM    
Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston, QLD, Australia.
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MeSH Terms
Antigens, Polyomavirus Transforming / analysis*,  biosynthesis*
Antigens, Viral / biosynthesis
Blotting, Southern
COS Cells / metabolism
Cell Division / physiology
Cell Line / immunology*
Chloramphenicol O-Acetyltransferase / genetics
Hepatitis B Surface Antigens / biosynthesis
Hepatitis B virus / genetics
Hepatitis Delta Virus / genetics,  immunology
Liver / cytology*
Protein Biosynthesis
Virus Replication / physiology
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Antigens, Viral; 0/Hepatitis B Surface Antigens; EC O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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