Document Detail


The development and characterisation of a SV40 T-antigen positive cell line of human hepatic origin.
MedLine Citation:
PMID:  9128863     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
COS7 cells and other cell lines expressing the SV40 large T-antigen, have been of great benefit in transfection studies using transient expression vectors which contain the SV40 origin of replication, as these cells allow plasmid replication to a high copy number. As no T-antigen expressing cell line derived from a well characterised hepatocyte-like continuous cell line currently exists, the establishment of such a cell line for studies which require expression of hepatocyte-specific factors would be extremely useful. A HepG2-derived stable cell line (THT1) was therefore developed which demonstrates a high level of transfection efficiency whilst retaining hepatocyte-like features, such as the production of hepatitis B virus. The THT1 cell line displayed chromosomal integration of the SV40 T-antigen gene and nuclear expression of the antigen. The cell line also maintained the general morphological features of its parent cell line, and showed an increased rate of cell growth.
Authors:
T J Harvey; T B Macnaughton; E J Gowans
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virological methods     Volume:  65     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-06-23     Completed Date:  1997-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  67-74     Citation Subset:  IM    
Affiliation:
Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston, QLD, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Polyomavirus Transforming / analysis*,  biosynthesis*
Antigens, Viral / biosynthesis
Blotting, Southern
COS Cells / metabolism
Cell Division / physiology
Cell Line / immunology*
Chloramphenicol O-Acetyltransferase / genetics
Hepatitis B Surface Antigens / biosynthesis
Hepatitis B virus / genetics
Hepatitis Delta Virus / genetics,  immunology
Humans
Liver / cytology*
Protein Biosynthesis
Transfection
Virus Replication / physiology
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Antigens, Viral; 0/Hepatitis B Surface Antigens; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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