Document Detail


The development of PCR-based markers for the selection of eyespot resistance genes Pch1 and Pch2.
MedLine Citation:
PMID:  18483719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two eyespot resistance genes (Pch1 and Pch2) have been characterised in wheat. The potent resistance gene Pch1, transferred from Aegilops ventricosa, is located on the distal end of the long arm of chromosome 7D (7DL). Pch2 derives from the variety Cappelle Desprez and is located at the distal end of chromosome 7AL. The RFLP marker Xpsr121 and the endopeptidase isozyme allele Ep-D1b, are very closely linked to Pch1, probably due to reduced recombination in the region of the introgressed A. ventricosa segment. Pch2 is less closely linked to these markers but is thought to be closer to Xpsr121 than to Ep-A1b. In the present study simple sequence repeat (SSR) markers were integrated into the genetic map of a single chromosome (7D) recombinant (RVPM) population segregating for Pch1. Sequence-tagged-site (STS)-based assays were developed for Xpsp121 and a 7DL wheat EST containing a SSR. SSR markers Xwmc14 and Xbarc97 and the Xpsr121-derived marker co-segregated with Pch1 in the RVPM population. A single chromosome (7A) recombinant population segregating for Pch2 was screened for eyespot resistance and mapped using SSRs. QTL interval mapping closely associated Pch2 with the SSR marker Xwmc525.
Authors:
N H Chapman; C Burt; H Dong; P Nicholson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-16
Journal Detail:
Title:  TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik     Volume:  117     ISSN:  0040-5752     ISO Abbreviation:  Theor. Appl. Genet.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-11     Completed Date:  2008-10-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0145600     Medline TA:  Theor Appl Genet     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  425-33     Citation Subset:  IM    
Affiliation:
John Innes Centre, Norwich Research Park, Colney, Norwich, UK.
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MeSH Terms
Descriptor/Qualifier:
Cellulases / metabolism
Chromosome Mapping
Chromosomes, Plant / genetics
Expressed Sequence Tags
Genes, Plant*
Genetic Markers
Immunity, Innate / genetics*
Minisatellite Repeats / genetics
Models, Genetic
Plant Diseases / genetics*,  immunology*
Polymerase Chain Reaction / methods*
Quantitative Trait Loci
Selection, Genetic*
Sequence Deletion
Triticum / enzymology,  genetics*
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Genetic Markers; EC 3.2.1.-/Cellulases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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