Document Detail


The developing neuroepithelium in human embryonic and fetal brain studied with vimentin-immunocytochemistry.
MedLine Citation:
PMID:  2476946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The neuroepithelial cells, which constitute the primordium of the CNS, are potentially capable of generating neuronal and glial cell lineages concomitantly. The appearance and morphological development of vimentin-positive neuroepithelial cells in human embryonic and fetal brain (4-16 weeks) were studied with immunocytochemistry. In embryos aged 4-6 weeks, vimentin-reactivity was seen in all neuroepithelial cells, including those which exhibited mitotic figures. The distribution of reactivity changed according to a general developmental pattern, which commenced and proceeded temporally different in various regions of the CNS. All regions exhibited vimentin-positive neuroepithelial cells, the distribution and morphology of which gradually changed, resulting in lamination of the neural wall into two and subsequently three layers. The neocortex and midline raphe were the only regions to differ significantly from the general pattern. When reactivity to glial fibrillary acidic protein developed at 7-8 weeks, the distribution was very much like that of vimentin at the same stage. Reactivity to glial, neuronal and other cellular markers (S-100, neurofilament, neuron specific enolase, desmin, and cytokeratin) revealed different distributions. Although cells retaining vimentin beyond the ventricular zone stage are radial glial cells and presumptive fibrous astrocytes, it seems unlikely that vimentin is a marker for a distinct cell lineage during early CNS development. It is suggested that all neuroepithelial cells in vivo differentiate to a stage where they express vimentin, and that vimentin may have a functional role in cellular movements and during the interkinetic nuclear migration.
Authors:
M Stagaard; K Møllgård
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anatomy and embryology     Volume:  180     ISSN:  0340-2061     ISO Abbreviation:  Anat. Embryol.     Publication Date:  1989  
Date Detail:
Created Date:  1989-10-23     Completed Date:  1989-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505194     Medline TA:  Anat Embryol (Berl)     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  17-28     Citation Subset:  IM    
Affiliation:
Department of Medical Anatomy A, Panum Institute, Copenhagen N, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Brain / cytology,  embryology*,  metabolism
Brain Stem / cytology,  metabolism
Choroid Plexus / cytology,  metabolism
Desmin / metabolism
Diencephalon / cytology,  metabolism
Epithelial Cells
Epithelium / metabolism
Fetus / cytology,  metabolism*
Glial Fibrillary Acidic Protein / metabolism
Humans
Immunohistochemistry
Intermediate Filament Proteins / metabolism
Keratins / metabolism
Phosphopyruvate Hydratase / metabolism
S100 Proteins / metabolism
Spinal Cord / cytology,  metabolism
Telencephalon / cytology,  metabolism
Vimentin / metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Desmin; 0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/S100 Proteins; 0/Vimentin; 68238-35-7/Keratins; EC 4.2.1.11/Phosphopyruvate Hydratase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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