| The determination of urinary 3-methyladenine in humans as a potential monitor of exposure to methylating agents. | |
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MedLine Citation:
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PMID: 3594728 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The determination of urinary 3-methyladenine has been explored as a potential monitor of exposure to methylating carcinogens. A method based on the use of high resolution gas chromatography--mass spectrometry has been developed for measuring this methylated base in human urine. Urine is extracted by XAD-2 column chromatography and the extract purified by reverse phase C18 h.p.l.c. Derivatization with N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide yields the mono-tert-butyldimethylsilyl derivative of 3-methyladenine which has good gas chromatographic properties and is stable towards hydrolysis. The gas chromatographic separation was carried out using an SE-52 capillary column and quantitation made by electron impact selective ion monitoring mass spectrometry employing deuterium-labelled 3-methyladenine added initially to the urine as internal standard. Measurable levels (4.50-16.07 micrograms/24 h) of the methylated base have been found in control (i.e. nominally unexposed) human urines. |
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Authors:
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D E Shuker; E Bailey; A Parry; J Lamb; P B Farmer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Carcinogenesis Volume: 8 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1987 Jul |
Date Detail:
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Created Date: 1987-08-05 Completed Date: 1987-08-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 959-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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analogs & derivatives*,
urine Alkylating Agents / metabolism* Carcinogens / metabolism* DNA / metabolism Gas Chromatography-Mass Spectrometry Humans Male Methylation Monitoring, Physiologic / methods* |
| Chemical | |
Reg. No./Substance:
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0/Alkylating Agents; 0/Carcinogens; 5142-23-4/3-methyladenine; 73-24-5/Adenine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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