Document Detail


The determination of sex from forcibly removed hairs.
MedLine Citation:
PMID:  6502106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The determination of sex from forcibly removed hairs in forensic science laboratories has, in the past, been based almost entirely on the presence or absence of the Y chromosome in the cells of the hair root sheath. Since the human male genotype is XY and the female is XX, a technique was devised that permits root sheath cells to be stained sequentially for the Y and then the X chromosome using quinacrine mustard. Following staining, the Y and the X chromosome fluorescence were observed, at pH 5.5 and 3.0, respectively, by epifluorescence. The X and Y chromosome counts obtained for a single hair root specimen were reported as a Y-X (Y minus X) score. The results reported show that specimens from males gave positive Y-X score while specimens from females gave negative Y-X scores. Results of an age study and blind trials were also reported.
Authors:
J L Mudd
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of forensic sciences     Volume:  29     ISSN:  0022-1198     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-01-02     Completed Date:  1985-01-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1072-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Female
Hair / analysis*
Humans
Male
Quinacrine Mustard / diagnostic use
Sex Chromosomes / analysis
Sex Determination (Analysis)*
X Chromosome / analysis
Y Chromosome / analysis
Chemical
Reg. No./Substance:
4213-45-0/Quinacrine Mustard

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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